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Analysis of changes in gene expression and metabolic profiles induced by silica-coated magnetic nanoparticles
- Analysis of changes in gene expression and metabolic profiles induced by silica-coated magnetic nanoparticles
- Shim W.; Paik M.J.; Nguyen D.-T.; Lee J.-K.; Lee Y.; Kim J.-H.; Shin E.-H.; Kang J.S.; Jung H.-S.; Choi S.; Park S.; Shim J.S.; Lee G.
- Ewha Authors
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- ACS Nano
- ACS Nano vol. 6, no. 9, pp. 7665 - 7680
- SCI; SCIE; SCOPUS
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- Magnetic nanoparticles (MNPs) have proven themselves to be useful in biomedical research; however, previous reports were insufficient to address the potential dangers of nanoparticles. Here, we investigated gene expression and metabolic changes based on the microarray and gas chromatography-mass spectrometry with human embryo kidney 293 cells treated with MNPs@SiO 2(RITC), a silica-coated MNP containing Rhodamine B isothiocyanate (RITC). In addition, measurement of reactive oxygen species (ROS) and ATP analysis were performed to evaluate the effect of MNPs@SiO 2(RITC) on mitochondrial function. Compared to the nontreated control, glutamic acid was increased by more than 2.0-fold, and expression of genes related to the glutamic acid metabolic pathway was also disturbed in 1.0 μg/μL of MNPs@SiO 2(RITC)-treated cells. Furthermore, increases in ROS concentration and mitochondrial damage were observed in this MNPs@SiO 2(RITC) concentration. The organic acids related to the Krebs cycle were also disturbed, and the capacity of ATP synthesis was decreased in cell treated with an overdose of MNPs@SiO 2(RITC). Collectively, these results suggest that overdose (1.0 μg/μL) of MNPs caused transcriptomic and metabolic disturbance. In addition, we suggest that a combination of gene expression and metabolic profiles will provide more detailed and sensitive toxicological evaluation for nanoparticles. © 2012 American Chemical Society.
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