Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박성수 | - |
dc.date.accessioned | 2016-08-28T10:08:47Z | - |
dc.date.available | 2016-08-28T10:08:47Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.other | OAK-9239 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223028 | - |
dc.description.abstract | Magnetic nanoparticles (MNPs) have proven themselves to be useful in biomedical research; however, previous reports were insufficient to address the potential dangers of nanoparticles. Here, we investigated gene expression and metabolic changes based on the microarray and gas chromatography-mass spectrometry with human embryo kidney 293 cells treated with MNPs@SiO 2(RITC), a silica-coated MNP containing Rhodamine B isothiocyanate (RITC). In addition, measurement of reactive oxygen species (ROS) and ATP analysis were performed to evaluate the effect of MNPs@SiO 2(RITC) on mitochondrial function. Compared to the nontreated control, glutamic acid was increased by more than 2.0-fold, and expression of genes related to the glutamic acid metabolic pathway was also disturbed in 1.0 μg/μL of MNPs@SiO 2(RITC)-treated cells. Furthermore, increases in ROS concentration and mitochondrial damage were observed in this MNPs@SiO 2(RITC) concentration. The organic acids related to the Krebs cycle were also disturbed, and the capacity of ATP synthesis was decreased in cell treated with an overdose of MNPs@SiO 2(RITC). Collectively, these results suggest that overdose (1.0 μg/μL) of MNPs caused transcriptomic and metabolic disturbance. In addition, we suggest that a combination of gene expression and metabolic profiles will provide more detailed and sensitive toxicological evaluation for nanoparticles. © 2012 American Chemical Society. | - |
dc.language | English | - |
dc.title | Analysis of changes in gene expression and metabolic profiles induced by silica-coated magnetic nanoparticles | - |
dc.type | Article | - |
dc.relation.issue | 9 | - |
dc.relation.volume | 6 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 7665 | - |
dc.relation.lastpage | 7680 | - |
dc.relation.journaltitle | ACS Nano | - |
dc.identifier.doi | 10.1021/nn301113f | - |
dc.identifier.wosid | WOS:000309040600013 | - |
dc.identifier.scopusid | 2-s2.0-84866682731 | - |
dc.author.google | Shim W. | - |
dc.author.google | Paik M.J. | - |
dc.author.google | Nguyen D.-T. | - |
dc.author.google | Lee J.-K. | - |
dc.author.google | Lee Y. | - |
dc.author.google | Kim J.-H. | - |
dc.author.google | Shin E.-H. | - |
dc.author.google | Kang J.S. | - |
dc.author.google | Jung H.-S. | - |
dc.author.google | Choi S. | - |
dc.author.google | Park S. | - |
dc.author.google | Shim J.S. | - |
dc.author.google | Lee G. | - |
dc.contributor.scopusid | 박성수(9275920900;41262153700) | - |
dc.date.modifydate | 20230627101106 | - |