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dc.contributor.author신동해-
dc.date.accessioned2016-08-28T12:08:54Z-
dc.date.available2016-08-28T12:08:54Z-
dc.date.issued2008-
dc.identifier.issn1744-3091-
dc.identifier.otherOAK-4704-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/219975-
dc.description.abstractMethanococcus jannaschii has an mtr gene cluster expressing N 5-methyltetrahydromethanopterin:coenzyme M methyltransferase, which generates methane by reducing CO2 with H2 with concomitant energy production under strictly anaerobic conditions. Some methanogenic archaea also have an mtr gene-cluster homologue, the mtxXAH gene cluster. M. jannaschii has both an entire mtr gene cluster and a single mtxX gene instead of the whole mtxXAH gene cluster. A PSI-BLAST search, secondary-structure prediction and the absence of phosphotransacetylase activity in M. jannaschii strongly support the possibility that the MtxX protein constitutes a unique methyltransferase family. In this study, the MtxX protein from M. jannaschii has been cloned, expressed, purified and crystallized. Synchrotron data were collected to 2.9 Å from a crystal of selenomethionine-substituted MtxX protein. The crystal belonged to the primitive hexagonal space group P6122, with unit-cell parameters a = 54.9, b = 54.9, c = 341.1 Å, β = 120.0°. A full structure determination is under way in order to provide insight into the structure-function relationship of this protein. © International Union of Crystallography 2008.-
dc.languageEnglish-
dc.titlePreliminary structural studies on the MtxX protein from Methanococcus jannaschii-
dc.typeArticle-
dc.relation.issue4-
dc.relation.volume64-
dc.relation.indexSCOPUS-
dc.relation.startpage300-
dc.relation.lastpage303-
dc.relation.journaltitleActa Crystallographica Section F: Structural Biology and Crystallization Communications-
dc.identifier.doi10.1107/S1744309108007033-
dc.identifier.wosidWOS:000254473200019-
dc.identifier.scopusid2-s2.0-41949103684-
dc.author.googleShin D.H.-
dc.contributor.scopusid신동해(15920079700)-
dc.date.modifydate20170522111116-
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