Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 신동해 | - |
dc.date.accessioned | 2016-08-28T12:08:54Z | - |
dc.date.available | 2016-08-28T12:08:54Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.other | OAK-4704 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219975 | - |
dc.description.abstract | Methanococcus jannaschii has an mtr gene cluster expressing N 5-methyltetrahydromethanopterin:coenzyme M methyltransferase, which generates methane by reducing CO2 with H2 with concomitant energy production under strictly anaerobic conditions. Some methanogenic archaea also have an mtr gene-cluster homologue, the mtxXAH gene cluster. M. jannaschii has both an entire mtr gene cluster and a single mtxX gene instead of the whole mtxXAH gene cluster. A PSI-BLAST search, secondary-structure prediction and the absence of phosphotransacetylase activity in M. jannaschii strongly support the possibility that the MtxX protein constitutes a unique methyltransferase family. In this study, the MtxX protein from M. jannaschii has been cloned, expressed, purified and crystallized. Synchrotron data were collected to 2.9 Å from a crystal of selenomethionine-substituted MtxX protein. The crystal belonged to the primitive hexagonal space group P6122, with unit-cell parameters a = 54.9, b = 54.9, c = 341.1 Å, β = 120.0°. A full structure determination is under way in order to provide insight into the structure-function relationship of this protein. © International Union of Crystallography 2008. | - |
dc.language | English | - |
dc.title | Preliminary structural studies on the MtxX protein from Methanococcus jannaschii | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 64 | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 300 | - |
dc.relation.lastpage | 303 | - |
dc.relation.journaltitle | Acta Crystallographica Section F: Structural Biology and Crystallization Communications | - |
dc.identifier.doi | 10.1107/S1744309108007033 | - |
dc.identifier.wosid | WOS:000254473200019 | - |
dc.identifier.scopusid | 2-s2.0-41949103684 | - |
dc.author.google | Shin D.H. | - |
dc.contributor.scopusid | 신동해(57217374185) | - |
dc.date.modifydate | 20230208115524 | - |