Local delivery of growth factor from PLGA nanoparticles coated on titanium implant for enhanced bone integration

Abstract

The initial attachment of osteoblasts with implant surfaces is an essential event for osseointegration of dental implants. To maximize the effect of dental implant, one important approach for bone healing and growth is the use of growth factors. Though various methods have been investigated produce different carrier systems the pharmacodynamics in the release of growth factors, there are several limitations related to stability of growth factor. In this study, the effect of PLGA coating on anodized surface using electrospray with incorporated bFGF was investigated. To determine the effect of growth factor coating, three different dose of bFGF were delivered in machined and anodized titanium discs. The mechanical properties of surface coating and release characteristics of incorporated growth factor were examined in vitro. The characterization of coated surface was performed by using scanning electron microscopy (SEM) and bioactivity of growth factor was examined by cell attachment assay and ALPase test. In control materials, 50% burst release of bFGF was observed by 24hrs from PLGA nanoparticles and greater than 99% of the bFGF was released by 5days in vitro. The biological activity of released bFGF was confirmed at selected time-points using osteoblast-like HOS cell assay. In conclusion, the developed coating method, electrospray, represents controlled release profile of growth factor and good cellular response for guided tissue regeneration.;전기분사(electrospray)방법을 이용하여 Ti-금속으로 구성되고 산화피막처리된 치아용 임플란트의 표면에 약물을 코팅하는 방법을 개발하였다. 임플란트의 표면에 고분자와 성장인자를 나노 크기의 입자로 코팅하여 약물을 서방출하도록 하였으며, 정확한 코팅량을 알기 위하여 흡광도를 이용한 실험을 하였다. 함유된 성장인자의 용출실험과 조골세포의 초기 부착실험, 분화능에 관련된 실험을 통하여 제조된 임플란트가 생체친화성을 증가시켰음을 알게 되었으며, 이는 전기분사방법을 통한 코팅방법이 성장인자의 코팅에 매우 효과적이며 특히 치주용 임플란트의 코팅에 적합함을 확인하게 해준다.