Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 배윤수 | - |
dc.date.accessioned | 2024-08-30T16:31:25Z | - |
dc.date.available | 2024-08-30T16:31:25Z | - |
dc.date.issued | 2024 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.other | OAK-35491 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/269635 | - |
dc.description.abstract | Angiotensin II (AngII) induces the contraction and proliferation of vascular smooth muscle cells (VSMCs). AngII activates phospholipase C-β (PLC-β), thereby inducing Ca2+ mobilization as well as the production of reactive oxygen species (ROS). Since contraction is a unique property of contractile VSMCs, signaling cascades related to the proliferation of VSMCs may differ. However, the specific molecular mechanism that controls the contraction or proliferation of VSMCs remains unclear. AngII-induced ROS production, migration, and proliferation were suppressed by inhibiting PLC-β3, inositol trisphosphate (IP3) receptor, and NOX or by silencing PLC-β3 or NOX1 but not by NOX4. However, pharmacological inhibition or silencing of PLC-β3 or NOX did not affect AngII-induced VSMC contraction. Furthermore, the AngII-dependent constriction of mesenteric arteries isolated from PLC-β3∆SMC, NOX1−/−, NOX4−/− and normal control mice was similar. AngII-induced VSMC contraction and mesenteric artery constriction were blocked by inhibiting the L-type calcium channel Rho-associated kinase 2 (ROCK2) or myosin light chain kinase (MLCK). The activation of ROCK2 and MLCK was significantly induced in PLC-β3∆SMC mice, whereas the depletion of Ca2+ in the extracellular medium suppressed the AngII-induced activation of ROCK2, MLCK, and vasoconstriction. AngII-induced hypertension was significantly induced in NOX1−/− and PLC-β3∆SMC mice, whereas LCCA ligation-induced neointima formation was significantly suppressed in NOX1−/− and PLC-β3∆SMC mice. These results suggest that PLC-β3 is essential for vascular hyperplasia through NOX1-mediated ROS production but is nonessential for vascular constriction or blood pressure regulation. © The Author(s) 2024. | - |
dc.description.sponsorship | Springer Nature | - |
dc.language | English | - |
dc.title | Phospholipase C-β3 is dispensable for vascular constriction but indispensable for vascular hyperplasia | - |
dc.type | Article | - |
dc.relation.issue | 7 | - |
dc.relation.volume | 56 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 1620 | - |
dc.relation.lastpage | 1630 | - |
dc.relation.journaltitle | Experimental and Molecular Medicine | - |
dc.identifier.doi | 10.1038/s12276-024-01271-6 | - |
dc.identifier.wosid | WOS:001258256500009 | - |
dc.identifier.scopusid | 2-s2.0-85197413441 | - |
dc.author.google | Jin | - |
dc.author.google | Seo Yeon | - |
dc.author.google | Ha | - |
dc.author.google | Jung Min | - |
dc.author.google | Kum | - |
dc.author.google | Hye Jin | - |
dc.author.google | Ma | - |
dc.author.google | Ji Soo | - |
dc.author.google | Hong Koo | - |
dc.author.google | Song | - |
dc.author.google | Sang Heon | - |
dc.author.google | Yang | - |
dc.author.google | Yong Ryoul | - |
dc.author.google | Lee | - |
dc.author.google | Ho | - |
dc.author.google | Bae | - |
dc.author.google | Yoon Soo | - |
dc.author.google | Yamamoto | - |
dc.author.google | Masahiro | - |
dc.author.google | Suh | - |
dc.author.google | Pann-Ghill | - |
dc.author.google | Sun Sik | - |
dc.contributor.scopusid | 배윤수(15031067200) | - |
dc.date.modifydate | 20240830133922 | - |