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iMAX FRET (Information Maximized FRET) for Multipoint Single-Molecule Structural Analysis
- Title
- iMAX FRET (Information Maximized FRET) for Multipoint Single-Molecule Structural Analysis
- Authors
- Joshi; Bhagyashree S.; de Lannoy; Carlos; Howarth; Mark R.; Kim; Sung Hyun; Joo; Chirlmin
- Ewha Authors
- 주철민
- SCOPUS Author ID
- 주철민
![scopus](/images/layout/icon2.png)
- Issue Date
- 2024
- Journal Title
- Nano Letters
- ISSN
- 1530-6984
- Citation
- Nano Letters vol. 24, no. 28, pp. 8487 - 8494
- Keywords
- computational structure prediction; programmable DNA binding; single-molecule conformational analysis; single-molecule FRET; single-molecule structural analysis
- Indexed
- SCIE; SCOPUS
![scopus](/images/layout/scopus2.gif)
- Document Type
- Article
- Abstract
- Understanding the structure of biomolecules is vital for deciphering their roles in biological systems. Single-molecule techniques have emerged as alternatives to conventional ensemble structure analysis methods for uncovering new biology in molecular dynamics and interaction studies, yet only limited structural information could be obtained experimentally. Here, we address this challenge by introducing iMAX FRET, a one-pot method that allows ab initio 3D profiling of individual molecules using two-color FRET measurements. Through the stochastic exchange of fluorescent weak binders, iMAX FRET simultaneously assesses multiple distances on a biomolecule within a few minutes, which can then be used to reconstruct the coordinates of up to four points in each molecule, allowing structure-based inference. We demonstrate the 3D reconstruction of DNA nanostructures, protein quaternary structures, and conformational changes in proteins. With iMAX FRET, we provide a powerful approach to advance the understanding of biomolecular structure by expanding conventional FRET analysis to three dimensions. © 2024 The Authors. Published by American Chemical Society.
- DOI
- 10.1021/acs.nanolett.4c00447
- Appears in Collections:
- 자연과학대학 > 물리학전공 > Journal papers
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