Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 손아정 | - |
dc.contributor.author | 임현정 | - |
dc.date.accessioned | 2024-08-23T16:30:42Z | - |
dc.date.available | 2024-08-23T16:30:42Z | - |
dc.date.issued | 2024 | - |
dc.identifier.issn | 4565-6535 | - |
dc.identifier.other | OAK-35896 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/269249 | - |
dc.description.abstract | A multi-target aptamer assay was developed as a phthalic acid ester (PAE) panel to screen selected PAEs in plastic leachate samples. The panel comprises 13 PAEs (PAE-13), namely dimethyl phthalate, diethyl phthalate, di-n-butyl phthalate, di-n-hexyl phthalate, diisobutyl phthalate, diisononyl phthalate, diisodecyl phthalate, mono-2-ethylhexyl phthalate, di-2-ethylhexyl phthalate, diphenyl phthalate, butyl benzyl phthalate, dicyclohexyl phthalate, and phthalic acid. Herein, we proposed an aptamer assay using a newly truncated aptamer (20-mer) and the 7-aminoactinomycin D fluorophore, which selectively binds to guanine in single-stranded DNA, resulting in increased fluorescence intensity. The assay is highly selective for PAE-13 clusters. The selectivity of the assay was evaluated using 13 different PAEs and mixtures depending on the side chain structure. The quantitative detection of PAEs was demonstrated by adopting mixed PAE-13 simulants and achieved a limit of detection of ∼1.4 pg/mL. The repeatability and reproducibility of the assay were also evaluated by presenting acceptable coefficients of variation (%CV less than 10% and 15%, respectively). The performance of the assay was demonstrated by analyzing the plastic leachate samples, and the positive correlation (correlation coefficient, r = 0.985) was confirmed by comparing them with the total sum of individual PAE peak areas obtained by gas chromatography mass spectrometry analysis. © 2024 Elsevier Ltd | - |
dc.description.sponsorship | Elsevier Ltd | - |
dc.language | English | - |
dc.subject | 7-Aminoactinomycin D | - |
dc.subject | Aptamer | - |
dc.subject | Phthalic acid esters | - |
dc.subject | Plastic leachate | - |
dc.title | Multi-target aptamer assay for endocrine-disrupting phthalic acid ester panel screening in plastic leachates | - |
dc.type | Article | - |
dc.relation.volume | 359 | - |
dc.relation.index | SCOPUS | - |
dc.relation.journaltitle | Chemosphere | - |
dc.identifier.doi | 10.1016/j.chemosphere.2024.142366 | - |
dc.identifier.scopusid | 2-s2.0-85193437975 | - |
dc.author.google | Lim | - |
dc.author.google | Hyun Jeong | - |
dc.author.google | Song | - |
dc.author.google | Hyerin | - |
dc.author.google | Son | - |
dc.author.google | Ahjeong | - |
dc.contributor.scopusid | 손아정(23487252600) | - |
dc.contributor.scopusid | 임현정(56514464800) | - |
dc.date.modifydate | 20240823160414 | - |