Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 정수영 | * |
dc.date.accessioned | 2024-05-20T16:31:22Z | - |
dc.date.available | 2024-05-20T16:31:22Z | - |
dc.date.issued | 2024 | * |
dc.identifier.issn | 1661-6596 | * |
dc.identifier.other | OAK-34829 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/268500 | - |
dc.description.abstract | REV-ERBα and its paralog, REV-ERBβ, encoded by NR1D1 and NR1D2 genes, are key nuclear receptors that link the circadian timing system and metabolic homeostasis. Since heme is an endogenous ligand, REV-ERBs have been considered key components of the circadian molecular clock and can be pharmacologically targeted to treat various circadian rhythm-related diseases, such as cardiometabolic, inflammatory, and neuropsychiatric diseases, as well as cancer. REV-ERBs are believed to be functionally redundant and compensatory, although they often affect the expression of gene subsets in an isoform-specific manner. Therefore, this study aimed to identify the redundant and distinct roles of each isoform in controlling its target genes by comparing the transcriptome profiles of a panel of mutant U2OS human osteosarcoma cells in which either NR1D1 or NR1D2 was ablated. Indeed, our transcriptomic analyses revealed that most REV-ERB-regulated genes are controlled by redundant or even additive actions. However, the RNA expression profiles of each single mutant cell line also provide strong evidence for isoform-dependent actions. For example, REV-ERBα is more responsible for regulating the NF-κΒ signaling pathway, whereas a group of extracellular matrix components requires REV-ERBβ to maintain their expression. We found that REV-ERBs have isoform-selective functions in the regulation of certain circadian output pathways despite their overlapping roles in the circadian molecular clock. Thus, the development of isoform-selective REV-ERB modulators can help treat metabolic disturbances and certain types of cancer. © 2024 by the authors. | * |
dc.language | English | * |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | * |
dc.subject | circadian clock | * |
dc.subject | circadian rhythm | * |
dc.subject | REV-ERBs | * |
dc.subject | transcriptome | * |
dc.subject | U2OS cell | * |
dc.title | Functional Characterization of Circadian Nuclear Receptors REV-ERBα and REV-ERBβ in Human Osteosarcoma Cell Cultures | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 25 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | International Journal of Molecular Sciences | * |
dc.identifier.doi | 10.3390/ijms25020770 | * |
dc.identifier.wosid | WOS:001150883200001 | * |
dc.identifier.scopusid | 2-s2.0-85183285783 | * |
dc.author.google | Cho | * |
dc.author.google | Hana | * |
dc.author.google | Yun | * |
dc.author.google | Ahee | * |
dc.author.google | Kim | * |
dc.author.google | Joohee | * |
dc.author.google | Park | * |
dc.author.google | Eunjeong | * |
dc.author.google | Jung | * |
dc.author.google | Jong-Wha | * |
dc.author.google | Chung | * |
dc.author.google | Sooyoung | * |
dc.author.google | Son | * |
dc.author.google | Gi Hoon | * |
dc.contributor.scopusid | 정수영(7404292716) | * |
dc.date.modifydate | 20240527165305 | * |