View : 120 Download: 0

Plasmonic Optical Wells-Based Enhanced Rate PCR

Title
Plasmonic Optical Wells-Based Enhanced Rate PCR
Authors
HanSeungyeonAnHyun JiKwakTaejinKimMiseolDongchoulLeeLuke P.ChoiInhee
Ewha Authors
Luke Pyung-Se Lee
Issue Date
2024
Journal Title
Nano Letters
ISSN
1530-6984JCR Link
Citation
Nano Letters vol. 24, no. 5, pp. 1738 - 1745
Keywords
light confinementPCRphotothermal heatingplasmonicspreconcentration
Publisher
American Chemical Society
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Rapid, sensitive, inexpensive point-of-care molecular diagnostics are crucial for the efficient control of spreading viral diseases and biosecurity of global health. However, the gold standard, polymerase chain reaction (PCR) is time-consuming and expensive and needs specialized testing laboratories. Here, we report a low-cost yet fast, selective, and sensitive Plasmonic Optical Wells-Based Enhanced Rate PCR: POWER-PCR. We optimized the efficient optofluidic design of 3D plasmonic optical wells via the computational simulation of light-to-heat conversion and thermophoretic convection in a self-created plasmonic cavity. The POWER-PCR chamber with a self-passivation layer can concentrate incident light to accumulate molecules, generate rapid heat transfer and thermophoretic flow, and minimize the quenching effect on the naked Au surface. Notably, we achieved swift photothermal cycling of nucleic acid amplification in POWER-PCR on-a-chip in 4 min 24 s. The POWER-PCR will provide an excellent solution for affordable and sensitive molecular diagnostics for precision medicine and preventive global healthcare. © 2024 American Chemical Society.
DOI
10.1021/acs.nanolett.3c04615
Appears in Collections:
ETC > ETC
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

BROWSE