View : 79 Download: 0

Transcriptional Changes in Radiation-Induced Lung Injury: A Comparative Analysis of Two Radiation Doses for Preclinical Research

Title
Transcriptional Changes in Radiation-Induced Lung Injury: A Comparative Analysis of Two Radiation Doses for Preclinical Research
Authors
FarhMohamed El-AgamyKimHyun-JinSang-YeonLeeJae-HeeHajeongCuiRonglanHanSoorimDong WookParkSunjooYoon-JinYun-SilSohnInsukChoJaeho
Ewha Authors
이윤실
SCOPUS Author ID
이윤실scopus
Issue Date
2024
Journal Title
International Journal of Molecular Sciences
ISSN
1661-6596JCR Link
Citation
International Journal of Molecular Sciences vol. 25, no. 7
Keywords
65 Gy and 75 Gyhistopathological analysisinflammation and fibrosis stagesmicro-computed tomographyradiation-induced lung injuryRNA sequencingstereotactic body radiation therapy
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
In a recent stereotactic body radiation therapy animal model, radiation pneumonitis and radiation pulmonary fibrosis were observed at around 2 and 6 weeks, respectively. However, the molecular signature of this model remains unclear. This study aimed to examine the molecular characteristics at these two stages using RNA-seq analysis. Transcriptomic profiling revealed distinct transcriptional patterns for each stage. Inflammatory response and immune cell activation were involved in both stages. Cell cycle processes and response to type II interferons were observed during the inflammation stage. Extracellular matrix organization and immunoglobulin production were noted during the fibrosis stage. To investigate the impact of a 10 Gy difference on fibrosis progression, doses of 45, 55, and 65 Gy were tested. A dose of 65 Gy was selected and compared with 75 Gy. The 65 Gy dose induced inflammation and fibrosis as well as the 75 Gy dose, but with reduced lung damage, fewer inflammatory cells, and decreased collagen deposition, particularly during the inflammation stage. Transcriptomic analysis revealed significant overlap, but differences were observed and clarified in Gene Ontology and KEGG pathway analysis, potentially influenced by changes in interferon-gamma-mediated lipid metabolism. This suggests the suitability of 65 Gy for future preclinical basic and pharmaceutical research connected with radiation-induced lung injury. © 2024 by the authors.
DOI
10.3390/ijms25073766
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

BROWSE