Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 권용억 | - |
dc.date.accessioned | 2024-05-16T16:30:06Z | - |
dc.date.available | 2024-05-16T16:30:06Z | - |
dc.date.issued | 2024 | - |
dc.identifier.issn | 0261-4189 | - |
dc.identifier.other | OAK-35171 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/268192 | - |
dc.description.abstract | Kinases that synthesize inositol phosphates (IPs) and pyrophosphates (PP-IPs) control numerous biological processes in eukaryotic cells. Herein, we extend this cellular signaling repertoire to viruses. We have biochemically and structurally characterized a minimalist inositol phosphate kinase (i.e., TvIPK) encoded by Terrestrivirus, a nucleocytoplasmic large (“giant”) DNA virus (NCLDV). We show that TvIPK can synthesize inositol pyrophosphates from a range of scyllo- and myo-IPs, both in vitro and when expressed in yeast cells. We present multiple crystal structures of enzyme/substrate/nucleotide complexes with individual resolutions from 1.95 to 2.6 Å. We find a heart-shaped ligand binding pocket comprising an array of positively charged and flexible side chains, underlying the observed substrate diversity. A crucial arginine residue in a conserved “G-loop” orients the γ-phosphate of ATP to allow substrate pyrophosphorylation. We highlight additional conserved catalytic and architectural features in TvIPK, and support their importance through site-directed mutagenesis. We propose that NCLDV inositol phosphate kinases may have assisted evolution of inositol pyrophosphate signaling, and we discuss the potential biogeochemical significance of TvIPK in soil niches. © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2024. | - |
dc.language | English | - |
dc.publisher | Springer Nature | - |
dc.subject | Cell Signaling | - |
dc.subject | Giant Virus | - |
dc.subject | Inositol Phosphate | - |
dc.subject | Kinase | - |
dc.subject | Phosphate Geochemistry | - |
dc.title | Biochemical and structural characterization of an inositol pyrophosphate kinase from a giant virus | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 43 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 462 | - |
dc.relation.lastpage | 480 | - |
dc.relation.journaltitle | EMBO Journal | - |
dc.identifier.doi | 10.1038/s44318-023-00005-0 | - |
dc.identifier.scopusid | 2-s2.0-85184135347 | - |
dc.author.google | Zong | - |
dc.author.google | Guangning | - |
dc.author.google | Desfougères | - |
dc.author.google | Yann | - |
dc.author.google | Portela-Torres | - |
dc.author.google | Paloma | - |
dc.author.google | Kwon | - |
dc.author.google | Yong-Uk | - |
dc.author.google | Saiardi | - |
dc.author.google | Adolfo | - |
dc.author.google | Stephen B. | - |
dc.author.google | Wang | - |
dc.author.google | Huanchen | - |
dc.author.google | Shears | - |
dc.contributor.scopusid | 권용억(7403459303) | - |
dc.date.modifydate | 20240516113209 | - |