Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 백지응 | * |
dc.date.accessioned | 2024-05-13T16:30:06Z | - |
dc.date.available | 2024-05-13T16:30:06Z | - |
dc.date.issued | 2024 | * |
dc.identifier.issn | 2772-9508 | * |
dc.identifier.other | OAK-35261 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/268098 | - |
dc.description.abstract | The behavior of stem cells is regulated by mechanical cues in their niche that continuously vary due to extracellular matrix (ECM) remodeling, pulsated mechanical stress exerted by blood flow, and/or cell migration. However, it is still unclear how dynamics of mechanical cues influence stem cell lineage commitment, especially in a 3D microenvironment where mechanosensing differs from that in a 2D microenvironment. In the present study, we investigated how temporally varying mechanical signaling regulates expression of the early growth response 1 gene (Egr1), which we recently discovered to be a 3D matrix-specific mediator of mechanosensitive neural stem cell (NSC) lineage commitment. Specifically, we temporally controlled the activity of Ras homolog family member A (RhoA), which is known to have a central role in mechanotransduction, using our previously developed Arabidopsis thaliana cryptochrome-2-based optoactivation system. Interestingly, pulsed RhoA activation induced Egr1 upregulation in stiff 3D gels only, whereas static light stimulation induced an increase in Egr1 expression across a wide range of 3D gel stiffnesses. Actin assembly inhibition limited Egr1 upregulation upon RhoA activation, implying that RhoA signaling requires an actin-involved process to upregulate Egr1. Consistently, static-light RhoA activation rather than pulsed-light activation restricted neurogenesis in soft gels. Our findings indicate that the dynamics of RhoA activation influence Egr1-mediated stem cell fate within 3D matrices in a matrix stiffness-dependent manner. © 2024 | * |
dc.language | English | * |
dc.publisher | Elsevier Ltd | * |
dc.subject | Early growth response (Egr1) | * |
dc.subject | Mechanosensing | * |
dc.subject | Neural stem cell (NSC) | * |
dc.subject | Optogenetics | * |
dc.subject | Ras homolog family member A (RhoA) signaling | * |
dc.title | Dynamic light-responsive RhoA activity regulates mechanosensitive stem cell fate decision in 3D matrices | * |
dc.type | Article | * |
dc.relation.volume | 160 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | Biomaterials Advances | * |
dc.identifier.doi | 10.1016/j.bioadv.2024.213836 | * |
dc.identifier.scopusid | 2-s2.0-85189757720 | * |
dc.author.google | Baek | * |
dc.author.google | Jieung | * |
dc.author.google | Kumar | * |
dc.author.google | Sanjay | * |
dc.author.google | Schaffer | * |
dc.author.google | David V. | * |
dc.date.modifydate | 20240607115435 | * |