Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 송은주 | * |
dc.contributor.author | 반은미 | * |
dc.date.accessioned | 2024-02-06T16:31:18Z | - |
dc.date.available | 2024-02-06T16:31:18Z | - |
dc.date.issued | 2023 | * |
dc.identifier.issn | 2405-8440 | * |
dc.identifier.other | OAK-34511 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/267058 | - |
dc.description.abstract | MicroRNAs (miRNAs) are small, non-coding RNAs crucial for gene regulation and implicated in various human diseases. Their potential as clinical prognostic and diagnostic biomarkers in biological fluids necessitates reliable detection methods. In this study, a combination of streptavidin-coupled magnetic beads and capillary electrophoresis with laser-induced fluorescence (CE-LIF) was used to extract and analyze plasma miRNAs. Specifically, miRNAs hybridized with a biotinylated fluorescent DNA probe were isolated from plasma using magnetic beads. These hybridized miRNAs were then directly injected into the CE-LIF system for analysis, eliminating the need for additional processing steps. Both the hybridization and bead-to-probe binding were executed concurrently, regulated by temperature and time. Through the optimization of magnetic bead extraction and CE-LIF conditions, we developed a highly sensitive assay for miR-21 quantification in plasma. The assay displayed remarkable linearity (R2 = 0.9975) within a 0.1–5 pM range and exhibited favorable precision (0.22–1.26 %) and accuracy (98.31–111.19 %). Importantly, we successfully detected endogenous miR-21 in plasma samples from both a lung cancer patient and healthy adults, revealing a 1.7-fold overexpression of miR-21 in lung cancer plasma relative to normal samples. Our findings suggest that this developed system offers a simple and sensitive approach for detecting endogenous miRNAs in plasma, showing its potential utility in disease diagnostics. To our knowledge, this is the first study to utilize CE-LIF for plasma miRNA detection. © 2023 The Authors | * |
dc.language | English | * |
dc.publisher | Elsevier Ltd | * |
dc.subject | Cancer biomarker | * |
dc.subject | Capillary electrophoresis | * |
dc.subject | Magnetic bead | * |
dc.subject | MicroRNA | * |
dc.title | Practical magnetic bead-based capillary electrophoresis with laser-induced fluorescence for detecting endogenous miRNA in plasma | * |
dc.type | Article | * |
dc.relation.issue | 12 | * |
dc.relation.volume | 9 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | Heliyon | * |
dc.identifier.doi | 10.1016/j.heliyon.2023.e22809 | * |
dc.identifier.wosid | WOS:001129842700001 | * |
dc.identifier.scopusid | 2-s2.0-85178349934 | * |
dc.author.google | Ban | * |
dc.author.google | Eunmi | * |
dc.author.google | Lim | * |
dc.author.google | Hee Ji | * |
dc.author.google | Kwon | * |
dc.author.google | Haejin | * |
dc.author.google | Song | * |
dc.author.google | Eun Joo | * |
dc.contributor.scopusid | 송은주(7101904210) | * |
dc.contributor.scopusid | 반은미(7006066577) | * |
dc.date.modifydate | 20240311110828 | * |