View : 170 Download: 0
The Inhibition Effect and Mechanism of Staurosporine Isolated from <i>Streptomyces</i> sp. SNC087 Strain on Nasal Polyp
- Title
- The Inhibition Effect and Mechanism of Staurosporine Isolated from <i>Streptomyces</i> sp. SNC087 Strain on Nasal Polyp
- Authors
- Choi, Grace; Lee, Eun-Young; Chung, Dawoon; Cho, Kichul; Yu, Woon-Jong; Nam, Sang-Jip; Park, Seong-Kook; Choi, Il-Whan
- Ewha Authors
- 남상집
- SCOPUS Author ID
- 남상집
- Issue Date
- 2024
- Journal Title
- MARINE DRUGS
- ISSN
- 1660-3397
- Citation
- MARINE DRUGS vol. 22, no. 1
- Keywords
- staurosporine; nasal polys; ECM proteins; myofibroblast differentiation; Streptomyces sp. SNC087
- Publisher
- MDPI
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- This study aims to explore the potential inhibition effects of staurosporine isolated from a Streptomyces sp. SNC087 strain obtained from seawater on nasal polyps. Staurosporine possesses antimicrobial and antihypertensive activities. This research focuses on investigating the effects of staurosporine on suppressing the growth and development of nasal polyps and elucidating the underlying mechanisms involved. The experimental design includes in vitro and ex vivo evaluations to assess the inhibition activity and therapeutic potential of staurosporine against nasal polyps. Nasal polyp-derived fibroblasts (NPDFs) were stimulated with TGF-beta 1 in the presence of staurosporine. The levels of alpha-smooth muscle actin (alpha-SMA), collagen type-I (Col-1), fibronectin, and phosphorylated (p)-Smad 2 were investigated using Western blotting. VEGF expression levels were analyzed in nasal polyp organ cultures treated with staurosporine. TGF-beta 1 stimulated the production of Col-1, fibronectin, and alpha-SMA and was attenuated by staurosporine pretreatment. Furthermore, these inhibitory effects were mediated by modulation of the signaling pathway of Smad 2 in TGF-beta 1-induced NPDFs. Staurosporine also inhibits the production of VEGF in ex vivo NP tissues. The findings from this study will contribute to a better understanding of staurosporine's role in nasal polyp management and provide insights into its mechanisms of action.
- DOI
- 10.3390/md22010039|http://dx.doi.org/10.3390/md22010039
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML