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Discovery, verification, and validation of walnut protein marker peptides using LC-MS approaches
- Title
- Discovery, verification, and validation of walnut protein marker peptides using LC-MS approaches
- Authors
- Kim; Kyungdo; Yourim; Lee; Hana; Chaeyoon; Sooyeon; Oh; Sangsuk; Park; Zee-Yong
- Ewha Authors
- 오상석
- SCOPUS Author ID
- 오상석
- Issue Date
- 2023
- Journal Title
- Food Chemistry
- ISSN
- 0308-8146
- Citation
- Food Chemistry vol. 429
- Keywords
- Food allergy; LC-MS; Validation; Walnut marker peptide
- Publisher
- Elsevier Ltd
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- A key requirement of liquid chromatography-mass spectrometry (LC-MS)-based allergenic food protein analysis methods is to use protein marker peptides with good analytical performances in LC-MS analysis of commercial processed foods. In this study, we developed a multi-stage walnut protein marker peptide selection strategy involving marker peptide discovery and verification and LC-MS validation of chemically equivalent stable isotope-labeled peptides. This strategy proposed three walnut protein marker peptides, including two new marker peptides. Our LC-MS-based walnut protein analysis method using the three stable isotope-labeled peptides showed acceptable linearity (R2 >0.99), matrix effects (coefficient of variation <±15%), sensitivity (limit of detection >0.3 pg/μL, limit of quantification >0.8 pg/μL), recovery (85.1–103.4%), accuracy, and precision (coefficient of variation <10%). In conclusion, our multi-stage marker peptide selection strategy effectively selects specific protein marker peptides for sensitive detection and absolute quantification of walnut proteins in LC-MS analysis of commercial processed foods. © 2023 Elsevier Ltd
- DOI
- 10.1016/j.foodchem.2023.136889
- Appears in Collections:
- 공과대학 > 식품생명공학과 > Journal papers
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