Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 남상집 | * |
dc.date.accessioned | 2023-07-27T16:31:15Z | - |
dc.date.available | 2023-07-27T16:31:15Z | - |
dc.date.issued | 2023 | * |
dc.identifier.issn | 1433-7851 | * |
dc.identifier.other | OAK-33727 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/265182 | - |
dc.description.abstract | Cihunamides A–D (1–4), novel antibacterial RiPPs, were isolated from volcanic-island-derived Streptomyces sp. The structures of 1–4 were elucidated by 1H, 13C, and 15N NMR, MS, and chemical derivatization; they contain a tetrapeptide core composed of WNIW, cyclized by a unique C−N linkage between two Trp units. Genome mining of the producer strain revealed two biosynthetic genes encoding a cytochrome P450 enzyme and a precursor peptide. Heterologous co-expression of the core genes demonstrated the biosynthesis of cihunamides through P450-mediated oxidative Trp-Trp cross-linking. Further bioinformatic analysis uncovered 252 homologous gene clusters, including that of tryptorubins, which possess a distinct Trp-Trp linkage. Cihunamides do not display the non-canonical atropisomerism shown in tryptorubins, which are the founding members of the “atropitide” family. Therefore, we propose to use a new RiPP family name, “bitryptides”, for cihunamides, tryptorubins, and their congeners, wherein the Trp-Trp linkages define the structural class rather than non-canonical atropisomerism. © 2023 Wiley-VCH GmbH. | * |
dc.language | English | * |
dc.publisher | John Wiley and Sons Inc | * |
dc.subject | Antibacterial Natural Products | * |
dc.subject | Biosynthesis | * |
dc.subject | Cytochrome P450 Enzymes | * |
dc.subject | RIPPs | * |
dc.subject | Structural Elucidation | * |
dc.title | Discovery and Biosynthesis of Cihunamides, Macrocyclic Antibacterial RiPPs with a Unique C−N Linkage Formed by CYP450 Catalysis | * |
dc.type | Article | * |
dc.relation.issue | 26 | * |
dc.relation.volume | 62 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | Angewandte Chemie - International Edition | * |
dc.identifier.doi | 10.1002/anie.202300998 | * |
dc.identifier.scopusid | 2-s2.0-85159067671 | * |
dc.author.google | An J.S. | * |
dc.author.google | Lee H. | * |
dc.author.google | Kim H. | * |
dc.author.google | Woo S. | * |
dc.author.google | Nam H. | * |
dc.author.google | Lee J. | * |
dc.author.google | Lee J.Y. | * |
dc.author.google | Nam S.-J. | * |
dc.author.google | Lee S.K. | * |
dc.author.google | Oh K.-B. | * |
dc.author.google | Kim S. | * |
dc.author.google | Oh D.-C. | * |
dc.contributor.scopusid | 남상집(57208839798) | * |
dc.date.modifydate | 20240220120010 | * |