Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 장선복 | * |
dc.date.accessioned | 2023-04-18T16:30:03Z | - |
dc.date.available | 2023-04-18T16:30:03Z | - |
dc.date.issued | 2023 | * |
dc.identifier.issn | 3051-1048 | * |
dc.identifier.other | OAK-33333 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/264956 | - |
dc.description.abstract | UV-damaged DNA-binding protein (UV-DDB) is a heterodimeric protein, consisting of DDB1 and DDB2 subunits, that works to recognize DNA lesions induced by UV damage during global genome nucleotide excision repair (GG-NER). Our laboratory previously discovered a non-canonical role for UV-DDB in the processing of 8-oxoG, by stimulating 8-oxoG glycosylase, OGG1, activity 3-fold, MUTYH activity 4-5-fold, and APE1 (apurinic/apyrimidinic endonuclease 1) activity 8-fold. 5-hydroxymethyl-deoxyuridine (5-hmdU) is an important oxidation product of thymidine which is removed by single-strand selective monofunctional DNA glycosylase (SMUG1). Biochemical experiments with purified proteins indicated that UV-DDB stimulates the excision activity of SMUG1 on several substrates by 4-5-fold. Electrophoretic mobility shift assays indicated that UV-DDB displaced SMUG1 from abasic site products. Single-molecule analysis revealed that UV-DDB decreases the half-life of SMUG1 on DNA by ∼8-fold. Immunofluorescence experiments demonstrated that cellular treatment with 5-hmdU (5 μM for 15 min), which is incorporated into DNA during replication, produces discrete foci of DDB2-mCherry, which co-localize with SMUG1-GFP. Proximity ligation assays supported a transient interaction between SMUG1 and DDB2 in cells. Poly(ADP)-ribose accumulated after 5-hmdU treatment, which was abrogated with SMUG1 and DDB2 knockdown. These data support a novel role for UV-DDB in the processing of the oxidized base, 5-hmdU. © 2023 The Author(s). | * |
dc.language | English | * |
dc.publisher | Oxford University Press | * |
dc.title | UV-DDB stimulates the activity of SMUG1 during base excision repair of 5-hydroxymethyl-2'-deoxyuridine moieties | * |
dc.type | Article | * |
dc.relation.issue | 10 | * |
dc.relation.volume | 51 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 4881 | * |
dc.relation.lastpage | 4898 | * |
dc.relation.journaltitle | Nucleic Acids Research | * |
dc.identifier.doi | 10.1093/nar/gkad206 | * |
dc.identifier.wosid | WOS:000952262700001 | * |
dc.identifier.scopusid | 2-s2.0-85162206432 | * |
dc.author.google | Jang S. | * |
dc.author.google | Raja S.J. | * |
dc.author.google | Roginskaya V. | * |
dc.author.google | Schaich M.A. | * |
dc.author.google | Watkins S.C. | * |
dc.author.google | Van Houten B. | * |
dc.contributor.scopusid | 장선복(23389133200) | * |
dc.date.modifydate | 20231218161651 | * |