View : 259 Download: 0
Increased Caveolin-2 Expression in Brain Endothelial Cells Promotes Age-Related Neuroinflammation
- Title
- Increased Caveolin-2 Expression in Brain Endothelial Cells Promotes Age-Related Neuroinflammation
- Authors
- Park, Hyunju; Shin, Jung A.; Lim, Jiwoo; Lee, Seulgi; Ahn, Jung-Hyuck; Kang, Jihee Lee; Choi, Youn-Hee
- Ewha Authors
- 이지희; 최윤희; 안정혁; 신정아; 박현주; 임지우
- SCOPUS Author ID
- 이지희; 최윤희; 안정혁; 신정아; 박현주; 임지우
- Issue Date
- 2022
- Journal Title
- MOLECULES AND CELLS
- ISSN
- 1016-8478
0219-1032
- Citation
- MOLECULES AND CELLS vol. 45, no. 12, pp. 950 - 962
- Keywords
- aging; cav2-; - mouse; caveolin-2; endothelial cell; neuroinflammation
- Publisher
- KOREAN SOC MOLECULAR &
CELLULAR BIOLOGY
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Aging is a major risk factor for common neurodegenerative diseases. Although multiple molecular, cellular, structural, and functional changes occur in the brain during aging, the involvement of caveolin-2 (Cav-2) in brain ageing remains unknown. We investigated Cav-2 expression in brains of aged mice and its effects on endothelial cells. The human umbilical vein endothelial cells (HUVECs) showed decreased THP-1 adhesion and infiltration when treated with Cav-2 siRNA compared to control siRNA. In contrast, Cav-2 overexpression increased THP-1 adhesion and infiltration in HUVECs. Increased expression of Cav-2 and iba-1 was observed in brains of old mice. Moreover, there were fewer iba-1-positive cells in the brains of aged Cav-2 knockout (KO) mice than of wild-type aged mice. The levels of several chemokines were higher in brains of aged wild-type mice than in young wild -type mice; moreover, chemokine levels were significantly lower in brains of young mice as well as aged Cav-2 KO mice than in their wild-type counterparts. Expression of PECAM1 and VE-cadherin proteins increased in brains of old wild -type mice but was barely detected in brains of young wild -type and Cav-2 KO mice. Collectively, our results suggest that Cav-2 expression increases in the endothelial cells of aged brain, and promotes leukocyte infiltration and age-associated neuroinflammation.
- DOI
- 10.14348/molcells.2022.0045
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML