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Single-stranded RNA adjuvant enhances the efficacy of 10-valent human papilloma virus-like particle vaccine
- Title
- Single-stranded RNA adjuvant enhances the efficacy of 10-valent human papilloma virus-like particle vaccine
- Authors
- Kwak, Hye Won; Shin, Wooseok; Baik, Kyunghwa; Kim, Minsun; Park, YongWook; Hong, So-Hee; Park, Hyo-Jung; Park, Hyeong-Jun; Bang, Yoo-Jin; Kim, Jae-Yong; Lee, Yu-Sun; Kim, In-Beom; Kim, Hong-Lim; Kim, Hun; Nam, Jae-Hwan
- Ewha Authors
- 홍소희
- SCOPUS Author ID
- 홍소희
- Issue Date
- 2022
- Journal Title
- MICROBIOLOGY AND IMMUNOLOGY
- ISSN
- 0385-5600
1348-0421
- Citation
- MICROBIOLOGY AND IMMUNOLOGY vol. 66, no. 11, pp. 529 - 537
- Keywords
- aluminum hydroxide; HPV virus like particle; long-lasting immunity; single-stranded RNA adjuvant; Th1 response
- Publisher
- WILEY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Following the development of various types of vaccines, the use of adjuvants to boost vaccine efficacy has become a focus of research. Aluminum hydroxide (alum), the most commonly used adjuvant, induces a certain immune response and ensures safety in human trials. However, alum mainly induces only a Th2 response; its Th1 response is weak. Thus, we previously developed a single-stranded ribose nucleic acid (ssRNA) adjuvant that induces a Th1 response through toll-like receptors. Here, we explored whether 10-valent human papilloma virus (HPV)-like particle (VLP) vaccine formulated with ssRNA adjuvant and alum helped to enhance immune response and maintained memory response. The mice were immunized intramuscularly twice at 2 week intervals and were inoculated 4 days after the second boost (after about 1 year). The antibody response and T cell activation were measured by Elispot, ELISA using harvested serum and splenocytes. The 10-valent HPV VLP vaccine formulated with ssRNA adjuvant and alum increased the antigen-specific immune response more than alum used alone. It increased each type-specific IgG1/IgG2a titer, and antigen-specific IFN-gamma cells. Furthermore, the ssRNA adjuvant with alum induced memory response. In memory response, each type-specific IgG1/IgG2c, IFN-gamma, and IL-6 cytokine, and neutralizing antibodies were increased by the ssRNA adjuvant with alum. Overall, the ssRNA adjuvant with alum induced memory responses and balanced Th1/Th2 responses. The ssRNA adjuvant and alum may help to enhance prophylactic vaccine efficacy.
- DOI
- 10.1111/1348-0421.13024
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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