Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 홍소희 | * |
dc.date.accessioned | 2023-01-04T16:31:17Z | - |
dc.date.available | 2023-01-04T16:31:17Z | - |
dc.date.issued | 2022 | * |
dc.identifier.issn | 0385-5600 | * |
dc.identifier.issn | 1348-0421 | * |
dc.identifier.other | OAK-32554 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/263030 | - |
dc.description.abstract | Following the development of various types of vaccines, the use of adjuvants to boost vaccine efficacy has become a focus of research. Aluminum hydroxide (alum), the most commonly used adjuvant, induces a certain immune response and ensures safety in human trials. However, alum mainly induces only a Th2 response; its Th1 response is weak. Thus, we previously developed a single-stranded ribose nucleic acid (ssRNA) adjuvant that induces a Th1 response through toll-like receptors. Here, we explored whether 10-valent human papilloma virus (HPV)-like particle (VLP) vaccine formulated with ssRNA adjuvant and alum helped to enhance immune response and maintained memory response. The mice were immunized intramuscularly twice at 2 week intervals and were inoculated 4 days after the second boost (after about 1 year). The antibody response and T cell activation were measured by Elispot, ELISA using harvested serum and splenocytes. The 10-valent HPV VLP vaccine formulated with ssRNA adjuvant and alum increased the antigen-specific immune response more than alum used alone. It increased each type-specific IgG1/IgG2a titer, and antigen-specific IFN-gamma cells. Furthermore, the ssRNA adjuvant with alum induced memory response. In memory response, each type-specific IgG1/IgG2c, IFN-gamma, and IL-6 cytokine, and neutralizing antibodies were increased by the ssRNA adjuvant with alum. Overall, the ssRNA adjuvant with alum induced memory responses and balanced Th1/Th2 responses. The ssRNA adjuvant and alum may help to enhance prophylactic vaccine efficacy. | * |
dc.language | English | * |
dc.publisher | WILEY | * |
dc.subject | aluminum hydroxide | * |
dc.subject | HPV virus like particle | * |
dc.subject | long-lasting immunity | * |
dc.subject | single-stranded RNA adjuvant | * |
dc.subject | Th1 response | * |
dc.title | Single-stranded RNA adjuvant enhances the efficacy of 10-valent human papilloma virus-like particle vaccine | * |
dc.type | Article | * |
dc.relation.issue | 11 | * |
dc.relation.volume | 66 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 529 | * |
dc.relation.lastpage | 537 | * |
dc.relation.journaltitle | MICROBIOLOGY AND IMMUNOLOGY | * |
dc.identifier.doi | 10.1111/1348-0421.13024 | * |
dc.identifier.wosid | WOS:000850329100001 | * |
dc.author.google | Kwak, Hye Won | * |
dc.author.google | Shin, Wooseok | * |
dc.author.google | Baik, Kyunghwa | * |
dc.author.google | Kim, Minsun | * |
dc.author.google | Park, YongWook | * |
dc.author.google | Hong, So-Hee | * |
dc.author.google | Park, Hyo-Jung | * |
dc.author.google | Park, Hyeong-Jun | * |
dc.author.google | Bang, Yoo-Jin | * |
dc.author.google | Kim, Jae-Yong | * |
dc.author.google | Lee, Yu-Sun | * |
dc.author.google | Kim, In-Beom | * |
dc.author.google | Kim, Hong-Lim | * |
dc.author.google | Kim, Hun | * |
dc.author.google | Nam, Jae-Hwan | * |
dc.contributor.scopusid | 홍소희(57197470752) | * |
dc.date.modifydate | 20240315135631 | * |