Polymetallic Hybrid Nanoplatform with Hyperthermia-Amplified Dual Enzyme-Like Activities for Efficient Speeded-Up Bacterially Infected Wound Healing

Abstract

Antibiotic-independent artificial nanozymes exhibit excellent biocatalytic activity, and can generate sufficient reactive oxygen species (ROS) for potential applications in the field of anti-infective therapy. However, single nanozymes often catalyze the production of ROS with low yield and low utilization, resulting in low antibacterial activity. To address the above refractory concern, a spike-like surface nanoparticles (CAC NPs) platform is built that possesses intrinsic dual enzyme-like (oxidase and peroxidase-like) activities and photothermal conversion capacity with near-infrared irradiation (NIR-I) to quickly capture and effectively eliminate the bacteria and biofilm. Upon NIR-I irradiation, CAC NPs have exerted an excellent biocatalytic antibacterial effect through a hyperthermia-amplified dual enzyme-like activities strategy. As predicted, the results show CAC NPs can eradicate bacteria in the subcutaneous tissues of mice and promote the healing of inflamed tissues with no significant side effects in vivo. Collectively, this work reveals the potential of NIR-assisted dual enzyme-like activities for antimicrobial therapy and provides broad prospects for antimicrobial drugs and strategies.