Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 손아정 | * |
dc.date.accessioned | 2023-01-04T16:31:17Z | - |
dc.date.available | 2023-01-04T16:31:17Z | - |
dc.date.issued | 2022 | * |
dc.identifier.issn | 0003-2654 | * |
dc.identifier.issn | 1364-5528 | * |
dc.identifier.other | OAK-32556 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/263028 | - |
dc.description.abstract | Contrary to the understanding that divalent cations only result in under-estimation of gene quantification via DNA hybridization-based assays, we have discovered that Mg2+ could cause either under or over-estimation at different concentrations. Its switchable inhibitory behavior is likely due to its rigid first solvation (hydrated) shell and hence it is inclined to form non-direct binding with DNA. At low concentrations, it caused under-estimation by occupying the hybridization sites. At high concentrations, it caused probe, signaling and target DNA to aggregate non-specifically via Coulomb forces. By quantifying target DNAs at a range of Mg2+ concentrations using a gene quantification assay (NanoGene assay), a Mg2+ inflection concentration of similar to 10(-3) M was observed for both target ssDNA and dsDNA. Field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDS), and Fourier transform infrared spectroscopy (FT-IR) were employed to observe Mg2+-induced non-specific binding in the complexes that mimicked the presence of target DNA. Together with two other divalent cations Ca2+ and Cu2+, they were further examined via zeta potential measurements as well as NanoGene assay. This study revealed the importance of Mg2+ in achieving accurate gene quantification. Through a better mechanistic understanding of this phenomenon, it will be possible to develop strategies to mitigate the impact of Mg2+ on DNA hybridization-based gene quantification. | * |
dc.language | English | * |
dc.publisher | ROYAL SOC CHEMISTRY | * |
dc.title | Switchable inhibitory behavior of divalent magnesium ion in DNA hybridization-based gene quantification | * |
dc.type | Article | * |
dc.relation.issue | 21 | * |
dc.relation.volume | 147 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 4845 | * |
dc.relation.lastpage | 4856 | * |
dc.relation.journaltitle | ANALYST | * |
dc.identifier.doi | 10.1039/d2an01164f | * |
dc.identifier.wosid | WOS:000857940100001 | * |
dc.author.google | Jin, Hyowon | * |
dc.author.google | Lim, Hyun Jeong | * |
dc.author.google | Liles, Mark R. | * |
dc.author.google | Chua, Beelee | * |
dc.author.google | Son, Ahjeong | * |
dc.contributor.scopusid | 손아정(23487252600) | * |
dc.date.modifydate | 20240322131724 | * |