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Label-free detection of leukemic myeloblasts in hyaluronic acid
- Title
- Label-free detection of leukemic myeloblasts in hyaluronic acid
- Authors
- Park S.; Kim H.; Woo M.; Kim M.
- Ewha Authors
- 김민석
- SCOPUS Author ID
- 김민석
- Issue Date
- 2022
- Journal Title
- Journal of Biological Engineering
- ISSN
- 1754-1611
- Citation
- Journal of Biological Engineering vol. 16, no. 1
- Keywords
- Microfluidic system; Motion microscopy; Myeloid leukemia; Vibration
- Publisher
- BioMed Central Ltd
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Chronic myeloid leukemia is generally required bone marrow biopsy for diagnosis. Although examining peripheral blood is less invasive, it has not been fully validated as a routine diagnostic test due to suboptimal sensitivity. To overcome this limitation, a number of methodologies based on microfluidics have been developed for sorting circulating tumor cells from peripheral blood of patients with leukemia. In order to develop a more convenient method, we designed an analysis protocol using motion microscopy that amplifies cellular micro motions in a captured video by re-rendering pixels to generate extreme magnified visuals. Intriguingly, no fluctuations around leukemic myeloblasts were observed with a motion microscope at any wavelength of 0–10 Hz. However, use of 0.05% hyaluronic acid, one type of non-newtonian fluid, demonstrated fluctuations around leukemic myeloblasts under conditions of 25 μm/s and 0.5–1.5 Hz with a motion microscope. Thus, the non-invasive detection of leukemic myeloblasts can offer a valuable supplementary diagnostic tool for assessment of drug efficacy for monitoring patients with chronic myeloid leukemia. © 2022, The Author(s).
- DOI
- 10.1186/s13036-022-00308-6
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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