View : 275 Download: 0
Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies
- Title
- Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies
- Authors
- Kim, Joo-Kyung; Yun, Hanool; Yeom, Chang-Hun; Kim, Eun-Jung; Kim, Wooseong; Lee, Chang-Soo; Kim, Byung-Gee; Jeong, Hee-Jin
- Ewha Authors
- 김우성
- SCOPUS Author ID
- 김우성
- Issue Date
- 2022
- Journal Title
- RSC ADVANCES
- ISSN
- 2046-2069
- Citation
- RSC ADVANCES vol. 12, no. 53, pp. 34660 - 34669
- Publisher
- ROYAL SOC CHEMISTRY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 10(3) to 10(5) CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources.
- DOI
- 10.1039/d2ra05694a
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML