Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 남상집 | * |
dc.date.accessioned | 2022-10-27T16:31:41Z | - |
dc.date.available | 2022-10-27T16:31:41Z | - |
dc.date.issued | 2022 | * |
dc.identifier.issn | 1660-3397 | * |
dc.identifier.other | OAK-32220 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/262788 | - |
dc.description.abstract | Cinnamoyl-containing nonribosomal peptides (CCNPs) form a unique family of actinobacterial secondary metabolites and display various biological activities. A new CCNP named epoxinnamide (1) was discovered from intertidal mudflat-derived Streptomyces sp. OID44. The structure of 1 was determined by the analysis of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) data along with a mass spectrum. The absolute configuration of 1 was assigned by the combination of advanced Marfey's method, (3)J(HH) and rotating-frame overhauser effect spectroscopy (ROESY) analysis, DP4 calculation, and genomic analysis. The putative biosynthetic pathway of epoxinnamide (1) was identified through the whole-genome sequencing of Streptomyces sp. OID44. In particular, the thioesterase domain in the nonribosomal peptide synthetase (NRPS) biosynthetic gene cluster was proposed as a bifunctional enzyme, which catalyzes both epimerization and macrocyclization. Epoxinnamide (1) induced quinone reductase (QR) activity in murine Hepa-1c1c7 cells by 1.6-fold at 5 mu M. It also exhibited effective antiangiogenesis activity in human umbilical vein endothelial cells (IC50 = 13.4 mu M). | * |
dc.language | English | * |
dc.publisher | MDPI | * |
dc.subject | cinnamoyl-containing nonribosomal peptide | * |
dc.subject | Streptomyces | * |
dc.subject | biosynthetic gene cluster | * |
dc.subject | bifunctional thioesterase | * |
dc.subject | quinone reductase | * |
dc.subject | angiogenesis | * |
dc.title | Epoxinnamide: An Epoxy Cinnamoyl-Containing Nonribosomal Peptide from an Intertidal Mudflat-Derived Streptomyces sp. | * |
dc.type | Article | * |
dc.relation.issue | 7 | * |
dc.relation.volume | 20 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | MARINE DRUGS | * |
dc.identifier.doi | 10.3390/md20070455 | * |
dc.identifier.wosid | WOS:000832271100001 | * |
dc.author.google | Kang, Sangwook | * |
dc.author.google | Han, Jaeho | * |
dc.author.google | Jang, Sung Chul | * |
dc.author.google | An, Joon Soo | * |
dc.author.google | Kang, Ilnam | * |
dc.author.google | Kwon, Yun | * |
dc.author.google | Nam, Sang-Jip | * |
dc.author.google | Shim, Sang Hee | * |
dc.author.google | Cho, Jang-Cheon | * |
dc.author.google | Lee, Sang Kook | * |
dc.author.google | Oh, Dong-Chan | * |
dc.contributor.scopusid | 남상집(57208839798) | * |
dc.date.modifydate | 20240220120010 | * |