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Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa
- Title
- Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa
- Authors
- Lim G.-M.; Kim J.-K.; Kim E.-J.; Lee C.-S.; Kim W.; Kim B.-G.; Jeong H.-J.
- Ewha Authors
- 김우성
- SCOPUS Author ID
- 김우성
- Issue Date
- 2022
- Journal Title
- BMC Biotechnology
- ISSN
- 1472-6750
- Citation
- BMC Biotechnology vol. 22, no. 1
- Keywords
- Enzyme-linked immunosorbent assay; HEK293F cells; Point-of-care testing; Pseudomonas aeruginosa; Recombinant antibody
- Publisher
- BioMed Central Ltd
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Pseudomonas aeruginosa (P. aeruginosa) is a major pathogen that causes nosocomial infections and often exhibits antibiotic resistance. Therefore, the development of an accurate method for detecting P. aeruginosa is required to control P. aeruginosa-related outbreaks. In this study, we established an enzyme-linked immunosorbent assay method for the sensitive detection of three P. aeruginosa strains, UCBPP PA14, ATCC 27853, and multidrug-resistant ATCC BAA-2108. We produced a recombinant antibody (rAb) against P. aeruginosa V‐antigen (PcrV), which is a needle tip protein of the type III secretion system of P. aeruginosa using mammalian cells with high yield and purity, and confirmed its P. aeruginosa binding efficiency. The rAb was paired with commercial anti-P. aeruginosa Ab for a sandwich ELISA, resulting in an antigen-concentration-dependent response with a limit of detection value of 230 CFU/mL. These results suggest that the rAb produced herein can be used for the sensitive detection of P. aeruginosa with a wide range of applications in clinical diagnosis and point-of-care testing. © 2022, The Author(s).
- DOI
- 10.1186/s12896-022-00751-9
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
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