View : 344 Download: 0
Rhodamine-thiourea Linked Naphthalimide Derivative to Image ATP in Mitochondria using Two Channels
- Title
- Rhodamine-thiourea Linked Naphthalimide Derivative to Image ATP in Mitochondria using Two Channels
- Authors
- Rim Lee, You; Kwon, Nahyun; Swamy, K. M. K.; Kim, Gyoungmi; Yoon, Juyoung
- Ewha Authors
- 윤주영
- SCOPUS Author ID
- 윤주영
- Issue Date
- 2022
- Journal Title
- CHEMISTRY-AN ASIAN JOURNAL
- ISSN
- 1861-4728
1861-471X
- Citation
- CHEMISTRY-AN ASIAN JOURNAL vol. 17, no. 15
- Keywords
- fluorescent probe for ATP; rhodamine; mitochondrial ATP; mitochondria targeting
- Publisher
- WILEY-V C H VERLAG GMBH
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Adenosine 5 '-triphosphate (ATP), synthesized in mitochondria, is an energy molecule in all living things. ATP not only serves as an energy source for protein synthesis and muscle contraction, but also as an important indicator for various diseases, such as Parkinson's disease, cardiovascular disease, and others. Accordingly, detection and sensing of ATP, especially in mitochondria, are important. In this study, a unique ring-opening process of rhodamine was coupled to recognition of ATP via introduction of a thiourea moiety, which was further linked to a naphthalimide group. A strong fluorescent emission at similar to 580 nm was accompanied by a color change from colorless to pink upon addition of ATP at pH 7.4. Fluorescent probe 1 successfully imaged mitochondrial ATP with a Pearson's coefficient of 0.8. In addition, green emission from the naphthalimide moiety at similar to 530 nm was observed without any change upon addition of ATP. This emission can be considered equivalent to an internal standard to utilize probe 1 as a dual-channel probe for ATP. Furthermore, probe 1 showed negligible cytotoxicity based on MTT assays.
- DOI
- 10.1002/asia.202200413
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML