Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박진병 | * |
dc.contributor.author | 차선신 | * |
dc.contributor.author | 송지원 | * |
dc.contributor.author | 우지민 | * |
dc.date.accessioned | 2022-08-11T16:30:59Z | - |
dc.date.available | 2022-08-11T16:30:59Z | - |
dc.date.issued | 2022 | * |
dc.identifier.issn | 0168-1656 | * |
dc.identifier.issn | 1873-4863 | * |
dc.identifier.other | OAK-31916 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/262280 | - |
dc.description.abstract | Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, which are involved in various functions including cell to cell signaling and protein excretion. Here, we have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for the degradation of beta-lactam antibiotics. This was exploited by targeting a beta-lactamase (i.e., CMY-10) into the OMVs of a hyper-vesiculating E. coli BL21(DE3) mutant. The CMY-10-containing OMVs, prepared from the E. coli mutant cultures, were able to hydrolyze beta-lactam ring of nitro-cefin and meropenem to a specific rate of 6.6 x 10(-8) and 3.9 x 10(-12) mu mol/min/mu m(3) of OMV, which is approximately 100 and 600-fold greater than those of E. coli-based whole-cell biocatalsyts. Furthermore, CMY-10, which was encapsulated in the engineered OMVs, was much more stable against temperature and acid stresses, as compared to free enzymes in aqueous phase. The OMV-based nano-scaled reaction system would be useful for the remediation of a variety of antibiotics pollution for food and agricultural industry. | * |
dc.language | English | * |
dc.publisher | ELSEVIER | * |
dc.subject | Outer membrane vesicles | * |
dc.subject | Antibiotics | * |
dc.subject | CMY-10 | * |
dc.subject | Nano-scale bioreactor | * |
dc.title | Engineering of a bacterial outer membrane vesicle to a nano-scale reactor for the biodegradation of beta-lactam antibiotics | * |
dc.type | Article | * |
dc.relation.volume | 356 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1 | * |
dc.relation.lastpage | 7 | * |
dc.relation.journaltitle | JOURNAL OF BIOTECHNOLOGY | * |
dc.identifier.doi | 10.1016/j.jbiotec.2022.07.003 | * |
dc.identifier.wosid | WOS:000836289700001 | * |
dc.identifier.scopusid | 2-s2.0-85134811854 | * |
dc.author.google | Woo, Ji-Min | * |
dc.author.google | Kim, Myeong-Yeon | * |
dc.author.google | Song, Ji-Won | * |
dc.author.google | Baeg, Yoonjin | * |
dc.author.google | Jo, Hye-Jin | * |
dc.author.google | Cha, Sun -Shin | * |
dc.author.google | Park, Jin-Byung | * |
dc.contributor.scopusid | 박진병(15036390700) | * |
dc.contributor.scopusid | 차선신(7201864593) | * |
dc.contributor.scopusid | 송지원(56118399000) | * |
dc.contributor.scopusid | 우지민(55274282300) | * |
dc.date.modifydate | 20240429134916 | * |