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Association between circulating bile acid alterations and nonalcoholic steatohepatitis independent of obesity and diabetes mellitus
- Title
- Association between circulating bile acid alterations and nonalcoholic steatohepatitis independent of obesity and diabetes mellitus
- Authors
- Jung, Youngae; Koo, Bo Kyung; Jang, Seo Young; Kim, Dain; Lee, Heeyeon; Lee, Dong Hyeon; Joo, Sae Kyung; Jung, Yong Jin; Park, Jeong Hwan; Yoo, Taekyeong; Choi, Murim; Lee, Min Kyung; Kang, Sang Won; Chang, Mee Soo; Kim, Won; Hwang, Geum-Sook|Innovative Target Exploration
- Ewha Authors
- 황금숙
- SCOPUS Author ID
- 황금숙
- Issue Date
- 2021
- Journal Title
- LIVER INTERNATIONAL
- ISSN
- 1478-3223
1478-3231
- Citation
- LIVER INTERNATIONAL vol. 41, no. 12, pp. 2892 - 2902
- Keywords
- aqueous metabolites; bile acid; fibrosis; nonalcoholic steatohepatitis; nonobese
- Publisher
- WILEY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Background and Aims Bile acid (BA) dysregulation is related to not only metabolic diseases but also nonalcoholic fatty liver disease (NAFLD). We investigated whether circulating BA levels are altered according to the histological severity of NAFLD independent of metabolic derangements. Methods Global metabolic profiling and targeted BA analysis using sera collected from biopsy-proven no-NAFLD (n = 67), nonalcoholic fatty liver (NAFL) (n = 99), and nonalcoholic steatohepatitis (NASH, n = 75) subjects were performed sequentially. Circulating metabolome analysis integrated with the hepatic transcriptome was performed to elucidate the mechanistic basis of altered circulating BA profiles after stratification by obesity (body mass index <= 25 kg/m(2)). Circulating BA alterations were also validated in an independent validation cohort (29 no-NAFLD, 70 NAFL and 37 NASH). Results Global profiling analysis showed that BA was the metabolite significantly altered in NASH compared to NAFL. Targeted BA analysis demonstrated that glyco-/tauro-conjugated primary BAs were commonly increased in nonobese and obese NASH, while unconjugated primary BAs increased only in nonobese NASH. These characteristic primary BA level changes were maintained even after stratification according to diabetes status and were replicated in the independent validation cohort. Compared to nonobese NAFL patients, nonobese NASH patients exhibited upregulated hepatic expression of CYP8B1. Conclusions BA metabolism is dysregulated as the histological severity of NAFLD worsens, independent of obesity and diabetes status; dysregulation is more prominent in nonobese NAFLD patients. Metabolome-driven omics approach provides new insight into our understanding of altered BA metabolism associated with individual phenotypes of NAFLD.
- DOI
- 10.1111/liv.15030
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
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