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Mesenchymal Stem Cell-Derived Exosomes Attenuate TLR7-Mediated Mast Cell Activation
- Title
- Mesenchymal Stem Cell-Derived Exosomes Attenuate TLR7-Mediated Mast Cell Activation
- Authors
- Cho K.-A.; Cha J.-E.; Kim J.; Kim Y.-H.; Ryu K.-H.; Woo S.-Y.
- Ewha Authors
- 유경하; 우소연; 조경아; 김유희
- SCOPUS Author ID
- 유경하; 우소연; 조경아; 김유희
- Issue Date
- 2022
- Journal Title
- Tissue Engineering and Regenerative Medicine
- ISSN
- 1738-2696
- Citation
- Tissue Engineering and Regenerative Medicine vol. 19, no. 1, pp. 117 - 129
- Keywords
- Exosomes; Mast cell; Mesenchymal stem cell; TLR7
- Publisher
- Korean Tissue Engineering and Regenerative Medicine Society
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Background:: Mast cells are immune sentinels in the skin that respond to a wide range of pathological and environmental stimuli; they owe their function to the expression of Toll-like receptors (TLRs). We previously found that tonsil-derived mesenchymal stem cells (T-MSCs) were able to effectively attenuate TLR7-mediated skin inflammation in mice, which was accompanied by an increase in mast cell number. The present study investigated whether T-MSC extracellular vesicles, such as exosomes, are able to regulate mast cell activation in response to TLR7 stimulation. Methods:: The HMC-1 human mast cell line was treated with a TLR7 agonist in the presence or absence of T-MSC exosomes, and the levels of expressed inflammatory cytokines were assessed. Additionally, mice were repeatedly injected with a TLR7 agonist with or without interval treatments with T-MSC exosomes and assessed dermal distribution of mast cells and related immune cells. Results:: We showed that T-MSC exosomes containing microRNAs that target inflammatory cytokines significantly reduced the expression of inflammatory cytokines in TLR7 agonist-treated HMC-1 cells. In addition, T-MSC exosomes inhibited the increase in the number of both dermal mast cells and CD14-positive cells in TLR7 agonist-treated mice. Conclusion:: Our data suggest that T-MSC exosomes have regulatory effects on mast cell activation under inflammatory conditions, including TLR7 stimulation. © 2021, The Korean Tissue Engineering and Regenerative Medicine Society.
- DOI
- 10.1007/s13770-021-00395-4
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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