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Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors
- Title
- Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors
- Authors
- Cho, Kyung-Ah; Choi, Da-Won; Kim, Yu-Hee; Kim, Jungwoo; Ryu, Kyung-Ha; Woo, So-Youn
- Ewha Authors
- 유경하; 우소연; 조경아; 김유희
- SCOPUS Author ID
- 유경하; 우소연; 조경아; 김유희
- Issue Date
- 2021
- Journal Title
- CELLS
- ISSN
- 2073-4409
- Citation
- CELLS vol. 10, no. 8
- Keywords
- mesenchymal stem cell; exosomes; skeletal muscle; activin A
- Publisher
- MDPI
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Skeletal muscle mass is decreased under a wide range of pathologic conditions. In particular, chemotherapy is well known for inducing muscle loss and atrophy. Previous studies using tonsil-derived mesenchymal stem cells (T-MSCs) or a T-MSC-conditioned medium showed effective recovery of total body weight in the chemotherapy-preconditioned bone marrow transplantation mouse model. This study investigated whether extracellular vesicles of T-MSCs, such as exosomes, are a key player in the recovery of body weight and skeletal muscle mass in chemotherapy-treated mice. T-MSC exosomes transplantation significantly decreased loss of total body weight and muscle mass in the busulfan-cyclophosphamide conditioning regimen in BALB/c recipient mice containing elevated serum activin A. Additionally, T-MSC exosomes rescued impaired C2C12 cell differentiation in the presence of activin A in vitro. We found that T-MSC exosomes possess abundant miR-145-5p, which targets activin A receptors, ACVR2A, and ACVR1B. Indeed, T-MSC exosomes rescue muscle atrophy both in vivo and in vitro via miR-145-5p dependent manner. These results suggest that T-MSC exosomes have therapeutic potential to maintain or improve skeletal muscle mass in various activin A elevated pathologic conditions.
- DOI
- 10.3390/cells10082169
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
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cells-10-02169-v3.pdf(7.62 MB)
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