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N-terminus-independent activation of c-Src via binding to a tetraspan(in) TM4SF5 in hepatocellular carcinoma is abolished by the TM4SF5 C-terminal peptide application
- Title
- N-terminus-independent activation of c-Src via binding to a tetraspan(in) TM4SF5 in hepatocellular carcinoma is abolished by the TM4SF5 C-terminal peptide application
- Authors
- Song, Haeng Eun; Lee, Yoonji; Kim, Eunmi; Cho, Chang Yun; Jung, Oisun; Lee, Doohyung; Lee, Eun Goo; Nam, Seo Hee; Kang, Minkyung; Macalino, Stephani Joy Y.; Kim, Ji Eon; Jung, Jae Woo; Kwon, Sung Won; Choi, Sun; Lee, Jung Weon
- Ewha Authors
- 최선; 이윤지
- SCOPUS Author ID
- 최선; 이윤지
- Issue Date
- 2021
- Journal Title
- THERANOSTICS
- ISSN
- 1838-7640
- Citation
- THERANOSTICS vol. 11, no. 16, pp. 8092 - 8111
- Keywords
- c-Src; metastasis; protein-protein interaction; PTPIB; TM4SF5
- Publisher
- IVYSPRING INT PUBL
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Active c-Src non-receptor tyrosine kinase localizes to the plasma membrane via N-terminal lipid modification. Membranous c-Src causes cancer initiation and progression. Even though transmembrane 4 L six family member 5 (TM4SF5), a tetraspan(in), can be involved in this mechanism, the molecular and structural influence of TM4SF5 on c-Src remains unknown. Methods: Here, we investigated molecular and structural details by which TM4SF5 regulated c-Src devoid of its N-terminus and how cell-penetrating peptides were able to interrupt c-Src activation via interference of c-Src-TM4SF5 interaction in hepatocellular carcinoma models. Results: The TM4SF5 C-terminus efficiently bound the c-Src SH1 kinase domain, efficiently to the inactively-closed form. The complex involved protein tyrosine phosphatase 1B able to dephosphorylate Tyr530. The c-Src SH1 domain alone, even in a closed form, bound TM4SF5 to cause c-Src Tyr419 and FAK Y861 phosphorylation. Homology modeling and molecular dynamics simulation studies predicted the directly interfacing residues, which were further validated by mutational studies. Cell penetration of TM4SF5 C-terminal peptides blocked the interaction of TM4SF5 with c-Src and prevented c-Src-dependent tumor initiation and progression in vivo. Conclusions: Collectively, these data demonstrate that binding of the TM4SF5 C-terminus to the kinase domain of inactive c-Src leads to its activation. Because this binding can be abolished by cell-penetrating peptides containing the TM4SF5 C-terminus, targeting this direct interaction may be an effective strategy for developing therapeutics that block the development and progression of hepatocellular carcinoma.
- DOI
- 10.7150/thno.58739
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
- Files in This Item:
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v11p8092.pdf(4.29 MB)
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