Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 차선신 | * |
dc.date.accessioned | 2021-08-12T16:31:26Z | - |
dc.date.available | 2021-08-12T16:31:26Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 1661-6596 | * |
dc.identifier.other | OAK-29544 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/258661 | - |
dc.description.abstract | Conventional human pluripotent stem cell (hPSC) cultures require high concentrations of expensive human fibroblast growth factor 2 (hFGF-2) for hPSC self-renewal and pluripotency in defined media for long-term culture. The thermal instability of the hFGF-2 mandates media change every day, which makes hPSC culture costly and cumbersome. Human DJ-1 (hDJ-1) can bind to and stimulate FGF receptor-1. In this study, for the first time, we have replaced hFGF-2 with hDJ-1 in the essential eight media and maintained the human embryonic stem cells (hESCs), H9, in the defined media at feeder-free condition. After more than ten passages, H9 in both groups still successfully maintained the typical hESC morphology and high protein levels of pluripotency markers, SSEA4, Tra1-60, Oct4, Nanog, and ALP. DNA microarray revealed that more than 97% of the 21,448 tested genes, including the pluripotency markers, Sox2, Nanog, Klf4, Lin28A, Lin28B, and Myc, have similar mRNA levels between the two groups. Karyotyping revealed no chromosome abnor-malities in both groups. They also differentiated sufficiently into three germ layers by forming in vitro EBs and in vivo teratomas. There were some variations in the RT-qPCR assay of several plu-ripotency markers. The proliferation rates and the mitochondria of both groups were also different. Taken together, we conclude that hDJ-1 can replace hFGF-2 in maintaining the self-renewal and the pluripotency of hESCs in feeder-free conditions. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | * |
dc.language | English | * |
dc.publisher | MDPI AG | * |
dc.subject | Defined media | * |
dc.subject | DJ-1 | * |
dc.subject | Feeder-free | * |
dc.subject | FGF-2 | * |
dc.subject | HPSC | * |
dc.title | Dj-1 can replace fgf-2 for long-term culture of human pluripotent stem cells in defined media and feeder-free condition | * |
dc.type | Article | * |
dc.relation.issue | 11 | * |
dc.relation.volume | 22 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | International Journal of Molecular Sciences | * |
dc.identifier.doi | 10.3390/ijms22115954 | * |
dc.identifier.wosid | WOS:000660203100001 | * |
dc.identifier.scopusid | 2-s2.0-85106888322 | * |
dc.author.google | Kim J. | * |
dc.author.google | Baek S. | * |
dc.author.google | Hong Y.-J. | * |
dc.author.google | de Paula M.N. | * |
dc.author.google | Prima M.J. | * |
dc.author.google | Oh Y.-M. | * |
dc.author.google | Cha S.-S. | * |
dc.author.google | Do J.-T. | * |
dc.author.google | Jang Y.-J. | * |
dc.author.google | Choe H. | * |
dc.contributor.scopusid | 차선신(7201864593) | * |
dc.date.modifydate | 20240429134916 | * |