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Anti-MRSA agent discovery using Caenorhabditis elegans-based high-throughput screening
- Title
- Anti-MRSA agent discovery using Caenorhabditis elegans-based high-throughput screening
- Authors
- Kim, Soo Min; Escorbar, Iliana; Lee, Kiho; Fuchs, Beth Burgwyn; Mylonakis, Eleftherios; Kim, Wooseong
- Ewha Authors
- 김우성
- SCOPUS Author ID
- 김우성
- Issue Date
- 2020
- Journal Title
- JOURNAL OF MICROBIOLOGY
- ISSN
- 1225-8873
1976-3794
- Citation
- JOURNAL OF MICROBIOLOGY vol. 58, no. 6, pp. 431 - 444
- Keywords
- antibiotic resistance; MRSA; Caenorhabditis elegans; high throughput screening; bacterial persisters; anti-infectives; host-pathogen interaction
- Publisher
- MICROBIOLOGICAL SOCIETY KOREA
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Review
- Abstract
- Staphylococcus aureus is a leading cause of hospital- and community-acquired infections. Despite current advances in antimicrobial chemotherapy, the infections caused by S. aureus remain challenging due to their ability to readily develop resistance. Indeed, antibiotic resistance, exemplified by methicillin-resistant S. aureus (MRSA) is a top threat to global health security. Furthermore, the current rate of antibiotic discovery is much slower than the rate of antibiotic-resistance development. It seems evident that the conventional in vitro bacterial growth-based screening strategies can no longer effectively supply new antibiotics at the rate needed to combat bacterial antibiotic-resistance. To overcome this antibiotic resistance crisis, screening assays based on host-pathogen interactions have been developed. In particular, the free-living nematode Caenorhabditis elegans has been used for drug screening against MRSA. In this review, we will discuss the general principles of the C. elegans-based screening platform and will highlight its unique strengths by comparing it with conventional antibiotic screening platforms. We will outline major hits from high-throughput screens of more than 100,000 small molecules using the C. elegans-MRSA infection assay and will review the mode-of-action of the identified hit compounds. Lastly, we will discuss the potential of a C. elegans-based screening strategy as a paradigm shift screening platform.
- DOI
- 10.1007/s12275-020-0163-8
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
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