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Extract of high hydrostatic pressure-treated danshen (Salvia miltiorrhiza) ameliorates atherosclerosis via autophagy induction
- Title
- Extract of high hydrostatic pressure-treated danshen (Salvia miltiorrhiza) ameliorates atherosclerosis via autophagy induction
- Authors
- Ko, Minjeong; Oh, Goo Taeg; Park, Jiyong; Kwon, Ho Jeong
- Ewha Authors
- 오구택
- SCOPUS Author ID
- 오구택
- Issue Date
- 2020
- Journal Title
- BMB REPORTS
- ISSN
- 1976-6696
1976-670X
- Citation
- BMB REPORTS vol. 53, no. 12, pp. 652 - 657
- Keywords
- Atherosclerosis; Autophagy; Cryptotanshinone; High hydrostatic pressure; Salvia miltiorrhiza
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY &
MOLECULAR BIOLOGY
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Danshen (Salvia miltiorrhiza) is a traditional medicinal plant widely used in Asian countries for its pharmacological activities (e.g., amelioration of cardiovascular diseases). In this study, we investigated the anti-atherosclerotic activity of raw danshen root extract prepared using high hydrostatic pressure (HHP) at 550 MPa for 5 min and hot water extraction. This method was useful for elimination of bacteria from cultured danshen plants and for better extraction yield of active principles. The HHP-treated danshen extract (HDE) inhibited proliferation of human umbilical vein endothelial cells (HUVECs) and induced autophagy that was assessed by LC3 conversion and p62 degradation. HDE suppressed foam cell formation in oxLDL-induced RAW264.7 macrophages; lysosomal activity simultaneously increased, measured by acridine orange staining. HDE also reduced atherosclerotic plaque development in vivo in apolipoprotein E knock-out (ApoE(-/-)) mice fed a high cholesterol diet. Taken together, these results indicated that HDE exhibited anti-atherosclerotic activity via autophagy induction.
- DOI
- 10.5483/BMBRep.2020.53.12.184
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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