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Efficient bioconversion of sugarcane bagasse into polyhydroxybutyrate (PHB) by Lysinibacillus sp. and its characterization
- Title
- Efficient bioconversion of sugarcane bagasse into polyhydroxybutyrate (PHB) by Lysinibacillus sp. and its characterization
- Authors
- Saratale, Rijuta Ganesh; Cho, Si Kyung; Saratale, Ganesh Dattatraya; Ghodake, Gajanan S.; Bharagava, Ram Naresh; Kim, Dong Su; Nair, Supriya; Shin, Han Seung
- Ewha Authors
- 김동수
- SCOPUS Author ID
- 김동수
- Issue Date
- 2021
- Journal Title
- BIORESOURCE TECHNOLOGY
- ISSN
- 0960-8524
1873-2976
- Citation
- BIORESOURCE TECHNOLOGY vol. 324
- Keywords
- Lysinibacillus sp. RGS; Sugarcane bagasse; Peracetic acid pretreatment; Poly(3-hydroxybutyrate); Saccharification yield; Corn steep liquor
- Publisher
- ELSEVIER SCI LTD
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- In this study, Lysinibacillus sp. RGS was evaluated to synthesize polyhydroxybutyrate (PHB) from a broad range of pure carbon sources and residual sugars of chemically pretreated sugarcane bagasse (SCB) hydrolysates. Effects of supplementation of nutrients and various experimental variables to enhance PHB accumulation were investigated. Results of optimized parameters were identified as 48 h, 37 degrees C, pH 7; inoculums concentration (2.5% v/ v) and shaking condition (100 rpm). Growth kinetics and bioprocess parameters of Lysinibacillus sp. using SCB hydrolysates with corn steep liquor (2%) accounted for the maximum cell growth (8.65 g/L) and PHA accumulation (61.5%) with PHB titer of (5.31 g/L) under optimal conditions. The produced biopolymer was studied by Fourier Transform Infrared (FTIR) spectroscopy and the results revealed the obtained to be PHB. Thus Lysinibaciluus sp. exhibits high potential in industrial scale manufacture of PHB using SCB as an inexpensive substrate.
- DOI
- 10.1016/j.biortech.2021.124673
- Appears in Collections:
- 공과대학 > 환경공학과 > Journal papers
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