Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김우성 | * |
dc.date.accessioned | 2020-12-03T16:30:09Z | - |
dc.date.available | 2020-12-03T16:30:09Z | - |
dc.date.issued | 2020 | * |
dc.identifier.issn | 1661-6596 | * |
dc.identifier.other | OAK-28270 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/255520 | - |
dc.description.abstract | Sphingosine-1-phophate (S1P) is a sphingolipid-derived signaling molecule that controls diverse cellular functions including cell growth, homeostasis, and stress responses. In a variety of metazoans, cytosolic S1P is transported into the extracellular space where it activates S1P receptors in a concentration-dependent manner. In the free-living nematode Caenorhabditis elegans, the spin-2 gene, which encodes a S1P transporter, is activated during Gram-positive or Gram-negative bacterial infection of the intestine. However, the role during infection of spin-2 and three additional genes in the C. elegans genome encoding other putative S1P transporters has not been elucidated. Here, we report an evolutionally conserved function for S1P and a non-canonical role for S1P transporters in the C. elegans immune response to bacterial pathogens. We found that mutations in the sphingosine kinase gene (sphk-1) or in the S1P transporter genes spin-2 or spin-3 decreased nematode survival after infection with Pseudomonas aeruginosa or Enterococcus faecalis. In contrast to spin-2 and spin-3, mutating spin-1 leads to an increase in resistance to P. aeruginosa. Consistent with these results, when wild-type C. elegans were supplemented with extracellular S1P, we found an increase in their lifespan when challenged with P. aeruginosa and E. faecalis. In comparison, spin-2 and spin-3 mutations suppressed the ability of S1P to rescue the worms from pathogen-mediated killing, whereas the spin-1 mutation had no effect on the immune-enhancing activity of S1P. S1P demonstrated no antimicrobial activity toward P. aeruginosa and Escherichia coli and only minimal activity against E. faecalis MMH594 (40 µM). These data suggest that spin-2 and spin-3, on the one hand, and spin-1, on the other hand, transport S1P across cellular membranes in opposite directions. Finally, the immune modulatory effect of S1P was diminished in C. elegans sek-1 and pmk-1 mutants, suggesting that the immunomodulatory effects of S1P are mediated by the p38 MAPK signaling pathway. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | * |
dc.language | English | * |
dc.publisher | MDPI AG | * |
dc.subject | Caenorhabditis elegans | * |
dc.subject | Immunity | * |
dc.subject | Pathogenic bacteria | * |
dc.subject | S1P | * |
dc.subject | S1P kinase | * |
dc.subject | S1P transporters | * |
dc.subject | Sphingosine-1-phosphate | * |
dc.title | In the model host caenorhabditis elegans, sphingosine-1-phosphate-mediated signaling increases immunity toward human opportunistic bacteria | * |
dc.type | Article | * |
dc.relation.issue | 21 | * |
dc.relation.volume | 21 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1 | * |
dc.relation.lastpage | 16 | * |
dc.relation.journaltitle | International Journal of Molecular Sciences | * |
dc.identifier.doi | 10.3390/ijms21217813 | * |
dc.identifier.wosid | WOS:000588963200001 | * |
dc.identifier.scopusid | 2-s2.0-85094143199 | * |
dc.author.google | Lee K. | * |
dc.author.google | Escobar I. | * |
dc.author.google | Jang Y. | * |
dc.author.google | Kim W. | * |
dc.author.google | Ausubel F.M. | * |
dc.author.google | Mylonakis E. | * |
dc.contributor.scopusid | 김우성(57201881427) | * |
dc.date.modifydate | 20240603112237 | * |