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Nucleoprotein vaccine induces cross-protective cytotoxic T lymphocytes against both lineages of influenza B virus
- Title
- Nucleoprotein vaccine induces cross-protective cytotoxic T lymphocytes against both lineages of influenza B virus
- Authors
- Lee S.-Y.; Kang J.-O.; Chang J.
- Ewha Authors
- 장준
- SCOPUS Author ID
- 장준
![scopus](/images/layout/icon2.png)
- Issue Date
- 2019
- Journal Title
- Clinical and Experimental Vaccine Research
- ISSN
- 2287-3651
- Citation
- Clinical and Experimental Vaccine Research vol. 8, no. 1, pp. 54 - 63
- Keywords
- Cross protective immunity; Cytotoxic T lymphocytes; Epitope; Influenza B virus; Nucleoproteins; Recombinant adenovirus
- Publisher
- Korean Vaccine Society
- Indexed
- SCOPUS; KCI
![scopus](/images/layout/scopus2.gif)
- Document Type
- Article
- Abstract
- Purpose: The influenza B virus diverges into two antigenically distinct lineages: B/Yamagata and B/Victoria. Influenza B is the dominant circulating virus during some influenza seasons, and recent data demonstrated that influenza A and B infection similarly cause severe clinical symptoms in hospitalized patients. Nucleoprotein (NP) is a good target for a universal influenza vaccine. This study investigated whether NP epitope variation within two lineages affects the dominant cytotoxic T lymphocyte (CTL) responses induced by vaccination and the resultant protective immunity. Materials and Methods: The NP of B/Yamagata/16/1988, the representative strain of the Yamagata lineage, includes a dominant CTL epitope, FSPIRITFL, while B/Shangdong/7/1997 from the Victoria lineage has one amino acid difference in this sequence, FSPIRVTFL. Two recombinant replication-deficient adenovirus (rAd)-vectored vaccines expressing either NP were prepared (rAd/B-NP(I) and rAd/B-NP(V), respectively) and administered to BALB/c mice intranasally. To examine the efficacy of vaccination, antibody responses, CTL responses, and morbidity/mortality after challenge were measured. Results: Both vaccines induce similar antibody and CD8 T-cell responses cross-reacting to both epitopes, and also confer cross-protection against both lineages regardless of amino acid difference. Conclusion: The rAd-vectored vaccine expressing the NP could be developed as universal influenza B vaccine which provides broader protection. © Korean Vaccine Society.
- DOI
- 10.7774/cevr.2019.8.1.54
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
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