Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이대기 | * |
dc.date.accessioned | 2020-08-13T16:30:11Z | - |
dc.date.available | 2020-08-13T16:30:11Z | - |
dc.date.issued | 2020 | * |
dc.identifier.issn | 1574-7891 | * |
dc.identifier.other | OAK-27257 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/254931 | - |
dc.description.abstract | Signal transducer and activator of transcription 3 (STAT3) regulates cell growth, cell survival, angiogenesis, metastasis of cancer cells, and cancer immune evasion by regulating gene expression as a transcription factor. However, the effect of STAT3 on translation is almost unknown. We demonstrated that STAT3 acts as a trans-acting factor for MLST8 gene expression and the protein level of mLST8, a core component of mechanistic target of rapamycin complex 1 and 2 (mTORC1/2), positively regulates the mTORC1/2 downstream pathways. Suppression of STAT3 by siRNA attenuated 4E-BP1 phosphorylation, cap-dependent translation, and cell proliferation in a variety of cancer cells. In HCT116 cells, STAT3 knockdown-induced decreases in 4E-BP1 and AKT phosphorylation levels were further attenuated by MLST8 knockdown or recovered by mLST8 overexpression. STAT3 knockdown-induced G2/M phase arrest was partially restored by co-knockdown of 4EBP1, and the attenuation of cell proliferation was enhanced by the expression of an mTORC1-mediated phosphorylation-defective mutant of 4E-BP1. ChIP and promoter mapping using a luciferase reporter assay showed that the −951 to −894 bp of MLST8 promoter seems to include STAT3-binding site. Overall, these results suggest that STAT3-driven MLST8 gene expression regulates cap-dependent translation through 4E-BP1 phosphorylation in cancer cells. © 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. | * |
dc.language | English | * |
dc.publisher | John Wiley and Sons Ltd | * |
dc.subject | 4E-BP1 | * |
dc.subject | cross-talk | * |
dc.subject | MLST8 | * |
dc.subject | mTORC1 | * |
dc.subject | STAT3 | * |
dc.title | STAT3-mediated MLST8 gene expression regulates cap-dependent translation in cancer cells | * |
dc.type | Article | * |
dc.relation.issue | 8 | * |
dc.relation.volume | 14 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1850 | * |
dc.relation.lastpage | 1867 | * |
dc.relation.journaltitle | Molecular Oncology | * |
dc.identifier.doi | 10.1002/1878-0261.12735 | * |
dc.identifier.wosid | WOS:000543801500001 | * |
dc.identifier.scopusid | 2-s2.0-85087221485 | * |
dc.author.google | Lee H. | * |
dc.author.google | Chin H. | * |
dc.author.google | Kim H. | * |
dc.author.google | Jung H. | * |
dc.author.google | Lee D. | * |
dc.contributor.scopusid | 이대기(37047040400) | * |
dc.date.modifydate | 20231120165418 | * |