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Lipidomic analysis reveals therapeutic effects of Yijin-Tang on high-fat/high-cholesterol diet-induced obese mice

Title
Lipidomic analysis reveals therapeutic effects of Yijin-Tang on high-fat/high-cholesterol diet-induced obese mice
Authors
Lee, So MinLee, JueunKang, EunjungKim, Hye-LinHwang, Geum-SookJung, Jeeyoun
Ewha Authors
황금숙
SCOPUS Author ID
황금숙scopusscopus
Issue Date
2020
Journal Title
PHYTOMEDICINE
ISSN
0944-7113JCR Link

1618-095XJCR Link
Citation
PHYTOMEDICINE vol. 74
Keywords
Yijin(Erchen)-TangLipidomicsObesityUPLC-Q-TOF-mass spectrometryTraditional Chinese medicine
Publisher
ELSEVIER GMBH
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
Background: In Traditional Korean and Chinese medicine, the herbal remedy Yijin (Erchen)-Tang (YJT) is widely used to treat obesity-related disorders, and its therapeutic potential has been demonstrated in numerous studies. However, the systemic effect of YJT on obesity status and change of lipid metabolism by YJT still remains unknown. Purpose: This study aimed to investigate the therapeutic mechanism of the YJT on obesity by using lipidomics. Methods: To evaluate the effects of treatment with YJT on obesity, C57BL/6 J mice were fed a high-fat and high-cholesterol (HFHC, 40% fat and 1% cholesterol) diet for 8 weeks and treated them with YJT for an additional 6 weeks. We then performed untargeted lipidomic analysis using ultra-performance liquid chromatography/ quadrupole-time-of-flight mass spectrometry mass spectroscopy coupled with multivariate statistical analysis. Results: YJT ameliorated obesity induced systemic inflammation and improved insulin resistance. Additionally, YJT protected against HFHC-diet-induced hepatic inflammation. To explore specific changes in lipid metabolism associated with the therapeutic effects of YJT, we performed untargeted lipid profiling of the plasma. Partial least squares-discriminant analysis (PLS-DA) score plots showed that YJT altered the lipid metabolic pattern of HFHC mice. In particular, ceramides and triglycerides with saturated fatty acids and monounsaturated fatty acids were significantly changed by YJT, which were significantly associated with insulin resistance, the AGERAGE signaling pathway in diabetic complications and adipocytokine signaling pathway in pathway enrichment analysis. Thus, we analyzed the changes in adipocytes and adipokine caused by YJT, and confirmed that YJT alleviated adipocytes inflammation and macrophage infiltration, and reversed HFHC-induced alterations in leptin and adiponectin levels in adipose tissue and plasma. Conclusion: These data suggest that YJT ameliorates obesity-induced systemic inflammation and insulin resistance by regulating lipid metabolism, and demonstrated that lipidomic profiling is a useful method to investigate the therapeutic effects of herbal decoctions in traditional Korean and Chinese medicine.
DOI
10.1016/j.phymed.2019.152936
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자연과학대학 > 화학·나노과학전공 > Journal papers
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