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Toxic potential of Bacillus cereus isolated from fermented alcoholic beverages
- Title
- Toxic potential of Bacillus cereus isolated from fermented alcoholic beverages
- Authors
- Kim, Sun Ae; Park, Hyun Jin; Cho, Tae Jin; Rhee, Min Suk
- Ewha Authors
- 김선애
- SCOPUS Author ID
- 김선애
- Issue Date
- 2020
- Journal Title
- FOOD RESEARCH INTERNATIONAL
- ISSN
- 0963-9969
1873-7145
- Citation
- FOOD RESEARCH INTERNATIONAL vol. 137
- Keywords
- Fermented alcoholic beverage; Bacillus cereus; Toxin gene; Enterotoxin; Cereulide
- Publisher
- ELSEVIER
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Pathogenicity of Bacillus cereus is associated with the production of various toxins. This study investigated the distribution of toxin genes encoding haemolysin BL (hblA, hblC, and hblD), nonhemolytic enterotoxin (nheA, nheB, and nheC), enterotoxin FM (entFM), cytotoxin K (cytK), and cereulide (ces) in 185 B. cereus strains isolated from draft beer, microbrewed beer, pasteurized beer, grape wine, other fruit wine, refined rice wine, traditional Korean pure liquor, and traditional Korean turbid rice wines. A total of 93.0% (172 isolates) of these isolates possessed at least one toxin gene. The nonhemolytic enterotoxin-encoding genes were highly prevalent in the isolates; the detection rate of enterotoxins was 91.4% for nheC, 81.6% for entFM, 62.7% for nheB, 57.3% for nheA, 53.0% for hblC, 48.6% for cytK, 36.8% for hblA, and 36.2% for hblD. Overall, 54.6% and 33.0% of strains carried the integrated Nhe-encoding gene cluster (nheA, nheB, nheC at the same time) and had the Hbl-encoding gene cluster, respectively. The cereulide synthetase gene was detected in only 2.2% of isolates. Toxin gene distribution patterns could be classified into 8 major profile clusters, and the most prevalent profile was the presence of enterotoxin genes only and no emetic toxin genes. Therefore, B. cereus in fermented alcoholic beverages was predominantly of the diarrhoeagenic type. Our results may provide important basic information when considering microbial standards and regulations for B. cereus in related products.
- DOI
- 10.1016/j.foodres.2020.109361
- Appears in Collections:
- 공과대학 > 식품생명공학과 > Journal papers
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