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Development of ultrasound aided chemical pretreatment methods to enrich saccharification of wheat waste biomass for polyhydroxybutyrate production and its characterization

Title
Development of ultrasound aided chemical pretreatment methods to enrich saccharification of wheat waste biomass for polyhydroxybutyrate production and its characterization
Authors
Saratale, Ganesh DattatrayaSaratale, Rijuta GaneshVarjani, SunitaCho, Si-KyungGhodake, Gajanan S.Kadam, AvinashMulla, Sikandar, IBharagava, Ram NareshKim, Dong-SuShin, Han Seung
Ewha Authors
김동수
SCOPUS Author ID
김동수scopus
Issue Date
2020
Journal Title
INDUSTRIAL CROPS AND PRODUCTS
ISSN
0926-6690JCR Link

1872-633XJCR Link
Citation
INDUSTRIAL CROPS AND PRODUCTS vol. 150
Keywords
Wheat waste biomassUltrasound pretreatmentPoly(3-hydroxybutyrate)Ralstonia eutropha (ATCC 17699)Biomass structureEnzymatic hydrolysis
Publisher
ELSEVIER
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Development of an effective pretreatment method is a vital stage to enhance biomass-based biopolymers production. The present study aims on the pretreatment of wheat waste biomass (WS) with alkaline and in combination with ultrasound (US) treatment to enhance enzymatic saccharification of WS into monomeric sugars. Effects of chemical dosage, substrate loading, US power and US pretreatment time on the pretreatment efficiency were systematically investigated. Under optimized conditions; US + NaOH pretreatment attained maximum 70% delignification of WS, with 84.5% hydrolysis yield, 90% glucose yield and 65% xylose yield after enzymatic hydrolysis (30 FPU/g of dry WS) which is substantially higher than the individual pretreatment. Analytical studies (XRD, FTIR, and SEM) revealed that the combined US + NaOH pretreatment can effectively destroyed the ultrastructure of biomass and improved the accessibility to hydrolyzing enzymes. The resulted enzymatic hydrolysates were utilized as a possible biomass feedstock for polyhydroxybutyrate (PHB) production using Ralstonia eutropha. Effects of supplementation of nutrients, stressing agents and initial concentration of US + NaOH pretreated WS hydrolysates (20 to 40 g/L) on PHB production were evaluated. The maximum 74% PHA accumulation, PHB titer of 7.85 g/L and yield of PHB about 0.441 g/g of reducing sugar production was obtained. Analytical characterization and thermal characteristics of extracted biopolymer showed the poperies of standard PHB. The foregoing results suggested the potential of WS as an alternate renewable resource for sustainable biopolymer production and enables WS waste biomass disposal concerns.
DOI
10.1016/j.indcrop.2020.112425
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엘텍공과대학 > 환경공학전공 > Journal papers
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