Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 차선신 | * |
dc.date.accessioned | 2020-04-06T16:30:05Z | - |
dc.date.available | 2020-04-06T16:30:05Z | - |
dc.date.issued | 2020 | * |
dc.identifier.issn | 0022-1759 | * |
dc.identifier.issn | 1872-7905 | * |
dc.identifier.other | OAK-26685 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/253715 | - |
dc.description.abstract | The specific recognition between a monoclonal antibody (mAb) and its epitope can be used in a tag system that has proved valuable in a wide range of biological applications. Herein, we describe a novel tag called RA-tag that is composed of a seven amino acid sequence (DIDLSRI) and recognized by a highly specific mAb, 47RA, against the bacterial toxin Vibrio vulnificus RtxA1/MARTX(Vv). By using recombinant proteins with the RA-tag at the N-terminal, C-terminal, or an internal site, we demonstrated that the tag system could be an excellent biological system for both protein purification and protein detection in enzyme-linked immunosorbent, Western blot, flow cytometry, and immunofluorescence staining analyses in Escherichia coll., mammalian cell lines, yeast, and plant. In addition, our RA-tag/47RA mAb combination showed high sensitivity and reliable affinity (K-D = 5.90 x 10(-8) M) when compared with conventional tags. Overall, our results suggest that the RA-tag system could facilitate the development of a broadly applicable tag system for biological research. | * |
dc.language | English | * |
dc.publisher | ELSEVIER | * |
dc.subject | Epitope tag | * |
dc.subject | Monoclonal antibody | * |
dc.subject | Immunodetection | * |
dc.subject | Affinity purification | * |
dc.title | Novel short peptide tag from a bacterial toxin for versatile applications | * |
dc.type | Article | * |
dc.relation.volume | 479 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | JOURNAL OF IMMUNOLOGICAL METHODS | * |
dc.identifier.doi | 10.1016/j.jim.2020.112750 | * |
dc.identifier.wosid | WOS:000520019300006 | * |
dc.identifier.scopusid | 2-s2.0-85078452386 | * |
dc.author.google | Lee, Tae Hee | * |
dc.author.google | Kim, Kwang Soo | * |
dc.author.google | Kim, Jin Hee | * |
dc.author.google | Jeong, Jae-Ho | * |
dc.author.google | Woo, Hye Ryun | * |
dc.author.google | Park, So Ra | * |
dc.author.google | Sohn, Myung-Ho | * |
dc.author.google | Lee, Hyeon Ju | * |
dc.author.google | Rhee, Joon Haeng | * |
dc.author.google | Cha, Sun-Shin | * |
dc.author.google | Hwang, Joo-Hee | * |
dc.author.google | Chung, Kyung Min | * |
dc.contributor.scopusid | 차선신(7201864593) | * |
dc.date.modifydate | 20240429134916 | * |