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Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy
- Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy
- Murfin, Lloyd C.; Weber, Maria; Park, Sang Jun; Kim, Won Tae; Lopez-Alled, Carlos M.; McMullin, Claire L.; Pradaux-Caggiano, Fabienne; Lyall, Catherine L.; Kociok-Kohn, Gabriele; Wenk, Jannis; Bull, Steven D.; Yoon, Juyoung; Kim, Hwan Myung; James, Tony D.; Lewis, Simon E.
- Ewha Authors
- SCOPUS Author ID
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- Journal Title
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY vol. 141, no. 49, pp. 19389 - 19396
- AMER CHEMICAL SOC
- SCIE; SCOPUS
- Document Type
- Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.
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