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Rab25 Deficiency Perturbs Epidermal Differentiation and Skin Barrier Function in Mice

Title
Rab25 Deficiency Perturbs Epidermal Differentiation and Skin Barrier Function in Mice
Authors
Jeong, HaengduengLim, Kyung-MinGoldenring, James R.Nam, Ki Taek
Ewha Authors
임경민
SCOPUS Author ID
임경민scopus
Issue Date
2019
Journal Title
BIOMOLECULES & THERAPEUTICS
ISSN
1976-9148JCR Link

2005-4483JCR Link
Citation
BIOMOLECULES & THERAPEUTICS vol. 27, no. 6, pp. 553 - 561
Keywords
Rab25SkinEpidermisEpidermal differentiationSkin proliferation
Publisher
KOREAN SOC APPLIED PHARMACOLOGY
Indexed
SCIE; SCOPUS; KCI WOS
Document Type
Article
Abstract
Rab25, a member of the Rab11 small GTPase family, is central to achieving cellular polarity in epithelial tissues. Rab25 is highly expressed in epithelial cells of various tissues including breast, vagina, cervix, the gastrointestinal tract, and skin. Rab25 plays key roles in tumorigenesis, mainly by regulating epithelial differentiation and proliferation. However, its role in skin physiology is relatively unknown. In this study, we demonstrated that Rab25 knock-out (KO) mice show a skin barrier dysfunction with high trans-epidermal water loss and low cutaneous hydration. To examine this observation, we investigated the histology and epidermal differentiation markers of the skin in Rab25 KO mice. Rab25 KO increased cell proliferation at the basal layer of epidermis, whereas the supra-basal layer remained unaffected. Ceramide, which is a critical lipid component for skin barrier function, was not altered by Rab25 KO in its distribution or amount, as determined by immunohistochemistry. Notably, levels of epidermal differentiation markers, including loricrin, involucrin, and keratins (5, 14, 1, and 10) increased prominently in Rab25 KO mice. In line with this, depletion of Rab25 with single hairpin RNA increased the expression of differentiation markers in a human keratinocyte cell line, HaCaT. Transcriptomic analysis of the skin revealed increased expression of genes associated with skin development, epidermal development, and keratinocyte differentiation in Rab25 KO mice. Collectively, these results suggested that Rab25 is involved in the regulation of epidermal differentiation and proliferation.
DOI
10.4062/biomolther.2019.125
Appears in Collections:
약학대학 > 약학과 > Journal papers
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