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Multi-Step Enzymatic Synthesis of 1,9-Nonanedioic Acid from a Renewable Fatty Acid and Its Application for the Enzymatic Production of Biopolyesters
- Title
- Multi-Step Enzymatic Synthesis of 1,9-Nonanedioic Acid from a Renewable Fatty Acid and Its Application for the Enzymatic Production of Biopolyesters
- Authors
- Lee, Hyun-Ju; Kang, Young-Seo; Kim, Chae-Yun; Seo, Eun-Ji; Pyo, Sang-Hyun; Park, Jin-Byung
- Ewha Authors
- 박진병
- SCOPUS Author ID
- 박진병
- Issue Date
- 2019
- Journal Title
- POLYMERS
- ISSN
- 2073-4360
- Citation
- POLYMERS vol. 11, no. 10
- Keywords
- whole-cell biocatalyst; alcohol/aldehyde dehydrogenase; alpha,omega-dicarboxylic acids; Corynebacterium glutamicum; polyesterification; polyester
- Publisher
- MDPI
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- 1,9-Nonanedioic acid is one of the valuable building blocks for producing polyesters and polyamides. Thereby, whole-cell biosynthesis of 1,9-nonanedioic acid from oleic acid has been investigated. A recombinant Corynebacterium glutamicum, expressing the alcohol/aldehyde dehydrogenases (ChnDE) of Acinetobacter sp. NCIMB 9871, was constructed and used for the production of 1,9-nonanedioic acid from 9-hydroxynonanoic acid, which had been produced from oleic acid. When 9-hydroxynonanoic acid was added to a concentration of 20 mM in the reaction medium, 1,9-nonanedioic acid was produced to 16 mM within 8 h by the recombinant C. glutamicum. The dicarboxylic acid was isolated via crystallization and then used for the production of biopolyester by a lipase. For instance, the polyesterification of 1,9-nonanedioic acid and 1,8-octanediol in diphenyl ether by the immobilized lipase B from Candida antarctica led to formation of the polymer product with the number-average molecular weight (M-n) of approximately 21,000. Thereby, this study will contribute to biological synthesis of long chain dicarboxylic acids and their application for the enzymatic production of long chain biopolyesters.
- DOI
- 10.3390/polym11101690
- Appears in Collections:
- 공과대학 > 식품생명공학과 > Journal papers
- Files in This Item:
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