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Microbial production of O-methylated flavanones from methylated phenylpropanoic acids in engineered Escherichia coli
- Microbial production of O-methylated flavanones from methylated phenylpropanoic acids in engineered Escherichia coli
- Cui, Heqing; Song, Myoung Chong; Lee, Ji Young; Yoon, Yeo Joon
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY vol. 46, no. 12, pp. 1707 - 1713
- Methylated flavanone; Microbial production; Homoeriodictyol; Phenylpropanoic acid; Escherichia coli
- SPRINGER HEIDELBERG
- SCIE; SCOPUS
- Document Type
- Methylated flavonoids possess improved bioactivities compared to their unmethylated counterparts. In this study, for the efficient production of O-methylated flavonoids from simple methylated phenylpropanoic acids, a recombinant Escherichia coli strain expressing 4-coumarate:coenzyme A ligase (4CL) from Oryza sativa and chalcone synthase (CHS) from Hordeum vulgare was constructed; this strain produced significant amount of homoeriodictyol (similar to 52 mg/L) as well as a few amount of hesperetin (0.4 mg/L), respectively, from ferulic acid and 4-methylcaffeic acid. This demonstrates, for the first time, that the scarce but valuable methylated flavanones can be successfully produced from methylated phenylpropanoic acids in a microbial host via an artificial biosynthetic pathway consisting of 4CL and CHS that can accept O-methylated precursors.
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