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Inhibition of SARS-CoV 3CL protease by flavonoids

Title
Inhibition of SARS-CoV 3CL protease by flavonoids
Authors
Jo, SeriKim, SuwonShin, Dong HaeKim, Mi-Sun
Ewha Authors
신동해김수원김미선
SCOPUS Author ID
신동해scopus; 김수원scopus
Issue Date
2020
Journal Title
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
ISSN
1475-6366JCR Link

1475-6374JCR Link
Citation
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY vol. 35, no. 1, pp. 145 - 151
Keywords
SARS-CoVSARS-CoV 3CLproflavonoidFRETinhibitory compounds
Publisher
TAYLOR &

FRANCIS LTD
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
There were severe panics caused by Severe Acute Respiratory Syndrome (SARS) and Middle-East Respiratory Syndrome-Coronavirus. Therefore, researches targeting these viruses have been required. Coronaviruses (CoVs) have been rising targets of some flavonoids. The antiviral activity of some flavonoids against CoVs is presumed directly caused by inhibiting 3C-like protease (3CLpro). Here, we applied a flavonoid library to systematically probe inhibitory compounds against SARS-CoV 3CLpro. Herbacetin, rhoifolin and pectolinarin were found to efficiently block the enzymatic activity of SARS-CoV 3CLpro. The interaction of the three flavonoids was confirmed using a tryptophan-based fluorescence method, too. An induced-fit docking analysis indicated that S1, S2 and S3 ' sites are involved in binding with flavonoids. The comparison with previous studies showed that Triton X-100 played a critical role in objecting false positive or overestimated inhibitory activity of flavonoids. With the systematic analysis, the three flavonoids are suggested to be templates to design functionally improved inhibitors.
DOI
10.1080/14756366.2019.1690480
Appears in Collections:
약학대학 > 약학과 > Journal papers
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