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Structural basis for the ATP-independent proteolytic activity of LonB proteases and reclassification of their AAA plus modules
- Title
- Structural basis for the ATP-independent proteolytic activity of LonB proteases and reclassification of their AAA plus modules
- Authors
- An, Young Jun; Na, Jung-Hyun; Kim, Myung-Il; Cha, Sun-Shin
- Ewha Authors
- 차선신
- SCOPUS Author ID
- 차선신
- Issue Date
- 2015
- Journal Title
- JOURNAL OF MICROBIOLOGY
- ISSN
- 1225-8873
1976-3794
- Citation
- JOURNAL OF MICROBIOLOGY vol. 53, no. 10, pp. 711 - 717
- Keywords
- AAA plus proteins; PS-1 insert; H2 insert; Ins1; Lon proteases; Thermococcus onnurineus NA1; ATP-independent proteolytic activity
- Publisher
- MICROBIOLOGICAL SOCIETY KOREA
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- Lon proteases degrade defective or denature proteins as well as some folded proteins for the control of cellular protein quality. There are two types of Lon proteases, LonA and LonB. Each consists of two functional components: a protease component and an ATPase associated with various cellular activities (AAA+ module). Here, we report the 2.03 A-resolution crystal structure of the isolated AAA+ module (iAAA+ module) of LonB from Thermococcus onnurineus NA1 (TonLonB). The iAAA+ module, having no bound nucleotide, adopts a conformation virtually identical to the ADP-bound conformation of AAA+ modules in the hexameric structure of TonLonB; this provides insights into the ATP-independent proteolytic activity observed in a LonB protease. Structural comparison of AAA+ modules between LonA and LonB revealed that the AAA+ modules of Lon proteases are separated into two distinct dades depending on their structural features. The AAA+ module of LonB belongs to the 'H2 & Ins1 insert dade (HINS dade)' defined for the first time in this study, while the AAA+ module of LonA is a member of the HCLR dade.
- DOI
- 10.1007/s12275-015-5417-5
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
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