Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신윤용 | * |
dc.contributor.author | 김대기 | * |
dc.date.accessioned | 2019-11-19T16:30:49Z | - |
dc.date.available | 2019-11-19T16:30:49Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1015-8987 | * |
dc.identifier.issn | 1421-9778 | * |
dc.identifier.other | OAK-25853 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/252016 | - |
dc.description.abstract | Background/Aims: Hypoxia is an environmental factor that aggravates liver fibrosis. HIF1 alpha activates hepatic stellate cells (HSCs) and increases transforming growth factor-beta (TGF-beta) signaling and the epithelial mesenchymal transition (EMT), accelerating the progression of fibrosis. We evaluated the anti-fibrotic therapeutic potential of a small-molecule inhibitor of TGF-beta type I receptor kinase, EW-7197, on HIF1 alpha-derived TGF-beta signaling in cholestatic liver fibrosis. Methods: We used a bile duct ligation (BDL)-operated rat model to characterize the role of HIF1 alpha-derived TGF-beta signaling in liver fibrosis. Cellular assays were performed in LX-2 cells (human immortalized HSCs). The anti-fibrotic effects of EW-7197 in liver tissues and HSCs were investigated via biochemical assays, immunohistochemistry (IHC), immunofluorescence (IF), chromatin immunoprecipitation (ChIP) assays, real-time PCR, and western blotting. Results: In our BDL rat model, orally administered EW-7197 inhibited fibrosis and attenuated HIF1 alpha-induced activation of HSCs and EMT in vivo. In addition, EW-7197 inhibited HIF1 alpha-derived HS[activation and expression of EMT markers in LX-2 cells in vitro. Conclusion: This study suggests that FW-7197 exhibits potential as a treatment for liver fibrosis because it inhibits HIF1 alpha-induced TGF-beta signaling. (C) 2016 The Author(s) Published by S. Karger AG, Basel | * |
dc.language | English | * |
dc.publisher | KARGER | * |
dc.subject | Cholestatic liver injury | * |
dc.subject | Hepatic stellate cell | * |
dc.subject | TGF-beta | * |
dc.subject | HIF1 alpha | * |
dc.subject | Epithelial mesenchymal transition | * |
dc.subject | EW-7197 | * |
dc.title | TGF-beta Type I Receptor Kinase Inhibitor EW-7197 Suppresses Cholestatic Liver Fibrosis by Inhibiting HIF1 alpha-Induced Epithelial Mesenchymal Transition | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 38 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 571 | * |
dc.relation.lastpage | 588 | * |
dc.relation.journaltitle | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | * |
dc.identifier.doi | 10.1159/000438651 | * |
dc.identifier.wosid | WOS:000371082300011 | * |
dc.author.google | Kim, Min-Jin | * |
dc.author.google | Park, Sang-A | * |
dc.author.google | Kim, Chun Hwa | * |
dc.author.google | Park, So-Yeon | * |
dc.author.google | Kim, Jung-Shin | * |
dc.author.google | Kim, Dae-Kee | * |
dc.author.google | Nam, Jeong-Seok | * |
dc.author.google | Sheen, Yhun Yhong | * |
dc.contributor.scopusid | 신윤용(6603872711) | * |
dc.contributor.scopusid | 김대기(35083694200) | * |
dc.date.modifydate | 20240118164500 | * |